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DOI | 10.1038/s41467-018-07107-y |
Structure determination from single molecule X-ray scattering with three photons per image | |
von Ardenne, Benjamin; Mechelke, Martin; Grubmueller, Helmut | |
2018-06-18 | |
发表期刊 | NATURE COMMUNICATIONS
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ISSN | 2041-1723 |
出版年 | 2018 |
卷号 | 9 |
文章类型 | Article |
语种 | 英语 |
国家 | Germany |
英文摘要 | Scattering experiments with femtosecond high-intensity free-electron laser pulses provide a new route to macromolecular structure determination. While currently limited to nanocrystals or virus particles, the ultimate goal is scattering on single biomolecules. The main challenges in these experiments are the extremely low signal-to-noise ratio due to the very low expected photon count per scattering image, often well below 100, as well as the random orientation of the molecule in each shot. Here we present a de novo correlation-based approach and show that three coherently scattered photons per image suffice for structure determination. Using synthetic scattering data of a small protein, we demonstrate nearatomic resolution of 3.3 A using 3.3 x 10(10) coherently scattered photons from 3.3 x 10(9 )images, which is within experimental reach. Further, our three-photon correlation approach is robust to additional noise from incoherent scattering; the number of disordered solvent molecules attached to the macromolecular surface should be kept small. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000435453200003 |
WOS关键词 | PHASE-RETRIEVAL ; ATOMIC-STRUCTURE ; DIFFRACTION ; RECONSTRUCTION ; ORIENTATION ; PARTICLES ; DYNAMICS ; SPARSE ; CRYSTALLOGRAPHY ; SYMMETRIES |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/204068 |
专题 | 资源环境科学 |
作者单位 | Max Planck Inst Biophys Chem, Dept Theoret & Computat Biophys, Fassberg 11, D-37077 Gottingen, Germany |
推荐引用方式 GB/T 7714 | von Ardenne, Benjamin,Mechelke, Martin,Grubmueller, Helmut. Structure determination from single molecule X-ray scattering with three photons per image[J]. NATURE COMMUNICATIONS,2018,9. |
APA | von Ardenne, Benjamin,Mechelke, Martin,&Grubmueller, Helmut.(2018).Structure determination from single molecule X-ray scattering with three photons per image.NATURE COMMUNICATIONS,9. |
MLA | von Ardenne, Benjamin,et al."Structure determination from single molecule X-ray scattering with three photons per image".NATURE COMMUNICATIONS 9(2018). |
条目包含的文件 | 条目无相关文件。 |
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