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DOI | 10.1038/s41467-018-06280-4 |
Reflective imaging improves spatiotemporal resolution and collection efficiency in light sheet microscopy | |
Wu, Yicong1; Kumar, Abhishek1; Smith, Corey2; Ardiel, Evan1; Chandris, Panagiotis1; Christensen, Ryan1; Rey-Suarez, Ivan1,3; Guo, Min1; Vishwasrao, Harshad D.4; Chen, Jiji4; Tang, Jianyong5; Upadhyaya, Arpita3,6,7; La Riviere, Patrick J.2,8; Shroff, Hari1,4,6,7,8 | |
2017-11-13 | |
发表期刊 | NATURE COMMUNICATIONS
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ISSN | 2041-1723 |
出版年 | 2017 |
卷号 | 8 |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | Light-sheet fluorescence microscopy (LSFM) enables high-speed, high-resolution, and gentle imaging of live specimens over extended periods. Here we describe a technique that improves the spatiotemporal resolution and collection efficiency of LSFM without modifying the underlying microscope. By imaging samples on reflective coverslips, we enable simultaneous collection of four complementary views in 250 ms, doubling speed and improving information content relative to symmetric dual-view LSFM. We also report a modified deconvolution algorithm that removes associated epifluorescence contamination and fuses all views for resolution recovery. Furthermore, we enhance spatial resolution (to < 300 nm in all three dimensions) by applying our method to single-view LSFM, permitting simultaneous acquisition of two high-resolution views otherwise difficult to obtain due to steric constraints at high numerical aperture. We demonstrate the broad applicability of our method in a variety of samples, studying mitochondrial, membrane, Golgi, and microtubule dynamics in cells and calcium activity in nematode embryos. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000414915900014 |
WOS关键词 | PLANE ILLUMINATION MICROSCOPY ; SPATIAL-RESOLUTION ; CALCIUM DYNAMICS ; C. ELEGANS ; T-CELLS ; EMBRYOS ; SUPERRESOLUTION ; CAPTURE ; FLUX |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/204007 |
专题 | 资源环境科学 |
作者单位 | 1.Natl Inst Biomed Imaging & Bioengn, Sect High Resolut Opt Imaging, NIH, Bethesda, MD 20892 USA; 2.Univ Chicago, Dept Radiol, Chicago, IL 60637 USA; 3.Univ Maryland, Biophys Program, College Pk, MD 02543 USA; 4.NIH, Adv Imaging & Microscopy Resource, Bethesda, MD 20892 USA; 5.JT Sci Consulting LLC, North Potomac, MD 20878 USA; 6.Univ Maryland, Dept Phys, College Pk, MD 20740 USA; 7.Univ Maryland, Inst Phys Sci & Technol, College Pk, MD 20740 USA; 8.Whitman Ctr, Marine Biol Lab, Woods Hole, MA 02543 USA |
推荐引用方式 GB/T 7714 | Wu, Yicong,Kumar, Abhishek,Smith, Corey,et al. Reflective imaging improves spatiotemporal resolution and collection efficiency in light sheet microscopy[J]. NATURE COMMUNICATIONS,2017,8. |
APA | Wu, Yicong.,Kumar, Abhishek.,Smith, Corey.,Ardiel, Evan.,Chandris, Panagiotis.,...&Shroff, Hari.(2017).Reflective imaging improves spatiotemporal resolution and collection efficiency in light sheet microscopy.NATURE COMMUNICATIONS,8. |
MLA | Wu, Yicong,et al."Reflective imaging improves spatiotemporal resolution and collection efficiency in light sheet microscopy".NATURE COMMUNICATIONS 8(2017). |
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