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DOI | 10.1038/s41467-018-04526-9 |
Two-step self-assembly of a spider silk molecular clamp | |
Rat, Charlotte1; Heiby, Julia C.1; Bunz, Jessica P.1,2; Neuweiler, Hannes1 | |
2018-11-14 | |
发表期刊 | NATURE COMMUNICATIONS
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ISSN | 2041-1723 |
出版年 | 2018 |
卷号 | 9 |
文章类型 | Article |
语种 | 英语 |
国家 | Germany; Sweden |
英文摘要 | Web spiders synthesize silk fibers of unique strength and extensibility through the controlled self-assembly of protein building blocks, so-called spidroins. The spidroin C-terminal domain is highly conserved and connects two polypeptide chains through formation of an all-helical, intertwined dimer. Here we use contact-induced fluorescence self-quenching and resonance energy transfer in combination with far-UV circular dichroism spectroscopy as three orthogonal structural probes to dissect the mechanism of folding and dimerization of a spidroin C-terminal domain from the major ampullate gland of the nursery web spider Euprosthenops australis. We show that helices forming the dimer core assemble very rapidly and fold on association. Subsequently, peripheral helices fold and dock slowly onto the preformed core. Lability of outer helices facilitates formation of a highly expanded, partially folded dimer. The high end-to-end distance of chain termini in the partially folded dimer suggests an extensibility module that contributes to elasticity of spider silk. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000450068100008 |
WOS关键词 | C-TERMINAL DOMAIN ; KNOTTED PROTEIN ; PH ; KINETICS ; SPIDROIN ; ASSOCIATION ; ELASTICITY ; THERMODYNAMICS ; DENATURATION ; CONSERVATION |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/203827 |
专题 | 资源环境科学 |
作者单位 | 1.Julius Maximilians Univ Wurzburg, Dept Biotechnol & Biophys, D-97074 Wurzburg, Germany; 2.AlbaNova Univ Ctr, Spiber Technol AB, SE-10691 Stockholm, Sweden |
推荐引用方式 GB/T 7714 | Rat, Charlotte,Heiby, Julia C.,Bunz, Jessica P.,et al. Two-step self-assembly of a spider silk molecular clamp[J]. NATURE COMMUNICATIONS,2018,9. |
APA | Rat, Charlotte,Heiby, Julia C.,Bunz, Jessica P.,&Neuweiler, Hannes.(2018).Two-step self-assembly of a spider silk molecular clamp.NATURE COMMUNICATIONS,9. |
MLA | Rat, Charlotte,et al."Two-step self-assembly of a spider silk molecular clamp".NATURE COMMUNICATIONS 9(2018). |
条目包含的文件 | 条目无相关文件。 |
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