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DOI | 10.1038/s41467-018-04173-0 |
Enhanced validation of antibodies for research applications | |
Edfors, Fredrik1,2; Hober, Andreas1,2; Linderback, Klas2; Maddalo, Gianluca1,2; Azimi, Alireza3; Sivertsson, Asa1,2; Tegel, Hanna2; Hober, Sophia2; Szigyarto, Cristina Al-Khalili1,2; Fagerberg, Linn1,2; von Feilitzen, Kalle1,2; Oksvold, Per1,2; Lindskog, Cecilia4; Forsstrom, Bjorn1,2; Uhlen, Mathias1,2,5 | |
2018-10-08 | |
发表期刊 | NATURE COMMUNICATIONS |
ISSN | 2041-1723 |
出版年 | 2018 |
卷号 | 9 |
文章类型 | Article |
语种 | 英语 |
国家 | Sweden; Denmark |
英文摘要 | There is a need for standardized validation methods for antibody specificity and selectivity. Recently, five alternative validation pillars were proposed to explore the specificity of research antibodies using methods with no need for prior knowledge about the protein target. Here, we show that these principles can be used in a streamlined manner for enhanced validation of research antibodies in Western blot applications. More than 6,000 antibodies were validated with at least one of these strategies involving orthogonal methods, genetic knockdown, recombinant expression, independent antibodies, and capture mass spectrometry analysis. The results show a path forward for efforts to validate antibodies in an application-specific manner suitable for both providers and users. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000446566000016 |
WOS关键词 | PROTEIN ; EXPRESSION |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/203816 |
专题 | 资源环境科学 |
作者单位 | 1.KTH Royal Inst Technol, Sci Life Lab, SE-17121 Stockholm, Sweden; 2.KTH Royal Inst Technol, Dept Prot Sci, SE-10691 Stockholm, Sweden; 3.Karolinska Inst, Karolinska Univ Hosp, Dept Oncol Pathol, SE-17177 Stockholm, Sweden; 4.Uppsala Univ, Dept Immunol Genet & Pathol, SE-75185 Uppsala, Sweden; 5.Tech Univ Denmark, Novo Nordisk Fdn Ctr Biosustainabil, DK-2970 Horsholm, Denmark |
推荐引用方式 GB/T 7714 | Edfors, Fredrik,Hober, Andreas,Linderback, Klas,et al. Enhanced validation of antibodies for research applications[J]. NATURE COMMUNICATIONS,2018,9. |
APA | Edfors, Fredrik.,Hober, Andreas.,Linderback, Klas.,Maddalo, Gianluca.,Azimi, Alireza.,...&Uhlen, Mathias.(2018).Enhanced validation of antibodies for research applications.NATURE COMMUNICATIONS,9. |
MLA | Edfors, Fredrik,et al."Enhanced validation of antibodies for research applications".NATURE COMMUNICATIONS 9(2018). |
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