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DOI | 10.1038/s41467-018-04195-8 |
Harnessing photoinduced electron transfer to optically determine protein sub-nanoscale atomic distances | |
Pantazis, Antonios1,2,3; Westerberg, Karin4; Althoff, Thorsten5; Abramson, Jeff5; Olcese, Riccardo1,5 | |
2018-11-09 | |
发表期刊 | NATURE COMMUNICATIONS
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ISSN | 2041-1723 |
出版年 | 2018 |
卷号 | 9 |
文章类型 | Article |
语种 | 英语 |
国家 | USA; Sweden |
英文摘要 | Proteins possess a complex and dynamic structure, which is influenced by external signals and may change as they perform their biological functions. We present an optical approach, distance-encoding photoinduced electron transfer (DEPET), capable of the simultaneous study of protein structure and function. An alternative to FRET-based methods, DEPET is based on the quenching of small conjugated fluorophores by photoinduced electron transfer: a reaction that requires contact of the excited fluorophore with a suitable electron donor. This property allows DEPET to exhibit exceptional spatial and temporal resolution capabilities in the range pertinent to protein conformational change. We report the first implementation of DEPET on human large-conductance K+ (BK) channels under voltage clamp. We describe conformational rearrangements underpinning BK channel sensitivity to electrical excitation, in conducting channels expressed in living cells. Finally, we validate DEPET in synthetic peptide length standards, to evaluate its accuracy in measuring sub-and near-nanometer intramolecular distances. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000449628200009 |
WOS关键词 | CA2+-ACTIVATED K+ CHANNEL ; VOLTAGE-CLAMP FLUOROMETRY ; ION-CHANNEL ; CONFORMATIONAL DYNAMICS ; BIMANE FLUORESCENCE ; POTASSIUM CHANNELS ; ENERGY-TRANSFER ; SLO1 CHANNEL ; ORGANIC-DYES ; BK CHANNELS |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/203802 |
专题 | 资源环境科学 |
作者单位 | 1.Univ Calif Los Angeles, Dept Anesthesiol & Perioperat Med, Div Mol Med, Los Angeles, CA 90095 USA; 2.Linkoping Univ, Dept Clin & Expt Med IKE, Div Neurobiol, S-58183 Linkoping, Sweden; 3.Linkoping Univ, Wallenberg Ctr Mol Med, S-58183 Linkoping, Sweden; 4.Amgen Inc, Thousand Oaks, CA 91320 USA; 5.Univ Calif Los Angeles, Dept Physiol, Los Angeles, CA 90095 USA |
推荐引用方式 GB/T 7714 | Pantazis, Antonios,Westerberg, Karin,Althoff, Thorsten,et al. Harnessing photoinduced electron transfer to optically determine protein sub-nanoscale atomic distances[J]. NATURE COMMUNICATIONS,2018,9. |
APA | Pantazis, Antonios,Westerberg, Karin,Althoff, Thorsten,Abramson, Jeff,&Olcese, Riccardo.(2018).Harnessing photoinduced electron transfer to optically determine protein sub-nanoscale atomic distances.NATURE COMMUNICATIONS,9. |
MLA | Pantazis, Antonios,et al."Harnessing photoinduced electron transfer to optically determine protein sub-nanoscale atomic distances".NATURE COMMUNICATIONS 9(2018). |
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