Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1038/s41467-018-03792-x |
Crystal structure of TcpK in complex with oriT DNA of the antibiotic resistance plasmid pCW3 | |
Traore, Daouda A. K.1,2; Wisniewski, Jessica A.3; Flanigan, Sarena F.1; Conroy, Paul J.1; Panjikar, Santosh1,4; Mok, Yee-Foong5; Lao, Carmen3; Griffin, Michael D. W.5; Adams, Vicki3; Rood, Julian I.3; Whisstock, James C.1,6,7 | |
2018-09-13 | |
发表期刊 | NATURE COMMUNICATIONS
![]() |
ISSN | 2041-1723 |
出版年 | 2018 |
卷号 | 9 |
文章类型 | Article |
语种 | 英语 |
国家 | Australia; Mali |
英文摘要 | Conjugation is fundamental for the acquisition of new genetic traits and the development of antibiotic resistance in pathogenic organisms. Here, we show that a hypothetical Clostridium perfringens protein, TcpK, which is encoded by the tetracycline resistance plasmid pCW3, is essential for efficient conjugative DNA transfer. Our studies reveal that TcpK is a member of the winged helix-turn-helix (wHTH) transcription factor superfamily and that it forms a dimer in solution. Furthermore, TcpK specifically binds to a nine-nucleotide sequence that is present as tandem repeats within the pCW3 origin of transfer (oriT). The X-ray crystal structure of the TcpK-TcpK box complex reveals a binding mode centered on and around the beta-wing, which is different from what has been previously shown for other wHTH proteins. Structure-guided mutagenesis experiments validate the specific interaction between TcpK and the DNA molecule. Additional studies highlight that the TcpK dimer is important for specific DNA binding. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000444494800016 |
WOS关键词 | CLOSTRIDIUM-PERFRINGENS STRAINS ; TETRACYCLINE RESISTANCE ; CONJUGATIVE TRANSFER ; NUCLEIC-ACIDS ; PROTEINS ; SYSTEM ; RECOGNITION ; VALIDATION ; REVEALS ; SERVER |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/203755 |
专题 | 资源环境科学 |
作者单位 | 1.Monash Univ, Monash Biomed Discovery Inst, Dept Biochem & Mol Biol, Infect & Immun Program, Clayton, Vic 3800, Australia; 2.USTTB, Fac Sci & Tech, BP E3206, Bamako, Mali; 3.Monash Univ, Monash Biomed Discovery Inst, Infect & Immun Program, Dept Microbiol, Clayton, Vic 3800, Australia; 4.Australian Synchrotron, Clayton, Vic 3168, Australia; 5.Univ Melbourne, Mol Sci & Biotechnol Inst Bio21, Biochem & Mol Biol, Parkville, Vic 3010, Australia; 6.Monash Univ, ARC Ctr Excellence Adv Mol Imaging, Clayton, Vic 3800, Australia; 7.Monash Univ, EMBL Australia, Clayton, Vic 3800, Australia |
推荐引用方式 GB/T 7714 | Traore, Daouda A. K.,Wisniewski, Jessica A.,Flanigan, Sarena F.,et al. Crystal structure of TcpK in complex with oriT DNA of the antibiotic resistance plasmid pCW3[J]. NATURE COMMUNICATIONS,2018,9. |
APA | Traore, Daouda A. K..,Wisniewski, Jessica A..,Flanigan, Sarena F..,Conroy, Paul J..,Panjikar, Santosh.,...&Whisstock, James C..(2018).Crystal structure of TcpK in complex with oriT DNA of the antibiotic resistance plasmid pCW3.NATURE COMMUNICATIONS,9. |
MLA | Traore, Daouda A. K.,et al."Crystal structure of TcpK in complex with oriT DNA of the antibiotic resistance plasmid pCW3".NATURE COMMUNICATIONS 9(2018). |
条目包含的文件 | 条目无相关文件。 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论