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DOI | 10.1038/s41467-018-03352-3 |
Nuclear lamina dysfunction triggers a germline stem cell checkpoint | |
Barton, Lacy J.1,2; Duan, Tingting1; Ke, Wenfan1,3; Luttinger, Amy1; Lovander, Kaylee E.1; Soshnev, Alexey A.1,4; Geyer, Pamela K.1 | |
2018-09-27 | |
发表期刊 | NATURE COMMUNICATIONS |
ISSN | 2041-1723 |
出版年 | 2018 |
卷号 | 9 |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | LEM domain (LEM-D) proteins are conserved components of the nuclear lamina (NL) that contribute to stem cell maintenance through poorly understood mechanisms. The Drosophila emerin homolog Otefin (Ote) is required for maintenance of germline stem cells (GSCs) and gametogenesis. Here, we show that ote mutants carry germ cell-specific changes in nuclear architecture that are linked to GSC loss. Strikingly, we found that both GSC death and gametogenesis are rescued by inactivation of the DNA damage response (DDR) kinases, ATR and Chk2. Whereas the germline checkpoint draws from components of the DDR pathway, genetic and cytological features of the GSC checkpoint differ from the canonical pathway. Instead, structural deformation of the NL correlates with checkpoint activation. Despite remarkably normal oogenesis, rescued oocytes do not support embryogenesis. Taken together, these data suggest that NL dysfunction caused by Otefin loss triggers a GSC-specific checkpoint that contributes to maintenance of gamete quality. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000445819400011 |
WOS关键词 | DNA-DAMAGE RESPONSE ; EMBRYONIC AXIS SPECIFICATION ; DOUBLE-STRAND BREAKS ; LEM-DOMAIN PROTEINS ; DROSOPHILA-MELANOGASTER ; LAMINOPATHIC DISEASES ; PROGEROID SYNDROMES ; REPLICATION STRESS ; MEMBRANE PROTEIN ; GENOME INTEGRITY |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/203752 |
专题 | 资源环境科学 |
作者单位 | 1.Univ Iowa, Dept Biochem, Iowa City, IA 52242 USA; 2.NYU, Sch Med, Skirball Inst, Dept Cell Biol, 540 First Ave, New York, NY 10016 USA; 3.Univ Virginia, Dept Biol, 485 McCormick Rd, Charlottesville, VA 22904 USA; 4.Rockefeller Univ, Lab Chromatin Biol & Epigenet, 1230 York Ave, New York, NY 10065 USA |
推荐引用方式 GB/T 7714 | Barton, Lacy J.,Duan, Tingting,Ke, Wenfan,et al. Nuclear lamina dysfunction triggers a germline stem cell checkpoint[J]. NATURE COMMUNICATIONS,2018,9. |
APA | Barton, Lacy J..,Duan, Tingting.,Ke, Wenfan.,Luttinger, Amy.,Lovander, Kaylee E..,...&Geyer, Pamela K..(2018).Nuclear lamina dysfunction triggers a germline stem cell checkpoint.NATURE COMMUNICATIONS,9. |
MLA | Barton, Lacy J.,et al."Nuclear lamina dysfunction triggers a germline stem cell checkpoint".NATURE COMMUNICATIONS 9(2018). |
条目包含的文件 | 条目无相关文件。 |
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