Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1038/s41467-018-03446-y |
FRET biosensor uncovers cAMP nano-domains at beta-adrenergic targets that dictate precise tuning of cardiac contractility | |
Surdo, Nicoletta C.1; Berrera, Marco2; Koschinski, Andreas1; Brescia, Marcella1; Machado, Matias R.3; Carr, Carolyn1; Wright, Peter4; Gorelik, Julia4; Morotti, Stefano5; Grandi, Eleonora5; Bers, Donald M.5; Pantano, Sergio3; Zaccolo, Manuela1,2 | |
2017-04-20 | |
发表期刊 | NATURE COMMUNICATIONS
![]() |
ISSN | 2041-1723 |
出版年 | 2017 |
卷号 | 8 |
文章类型 | Article |
语种 | 英语 |
国家 | England; Scotland; Uruguay; USA |
英文摘要 | Compartmentalized cAMP/PKA signalling is now recognized as important for physiology and pathophysiology, yet a detailed understanding of the properties, regulation and function of local cAMP/PKA signals is lacking. Here we present a fluorescence resonance energy transfer (FRET)-based sensor, CUTie, which detects compartmentalized cAMP with unprecedented accuracy. CUTie, targeted to specific multiprotein complexes at discrete plasmalemmal, sarcoplasmic reticular and myofilament sites, reveals differential kinetics and amplitudes of localized cAMP signals. This nanoscopic heterogeneity of cAMP signals is necessary to optimize cardiac contractility upon adrenergic activation. At low adrenergic levels, and those mimicking heart failure, differential local cAMP responses are exacerbated, with near abolition of cAMP signalling at certain locations. This work provides tools and fundamental mechanistic insights into subcellular adrenergic signalling in normal and pathological cardiac function. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000399582000001 |
WOS关键词 | TROPONIN-I PHOSPHORYLATION ; PROTEIN-KINASE-A ; HEART-FAILURE ; CYCLIC-AMP ; INTACT-CELLS ; CALCIUM ; TIME ; PHOSPHODIESTERASES ; MYOFILAMENTS ; RELAXATION |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/203722 |
专题 | 资源环境科学 |
作者单位 | 1.Univ Oxford, Dept Physiol Anat & Genet, Oxford OX1 3PT, England; 2.Univ Glasgow, Inst Neurosci & Psychol, Mol Pharmacol Ctr, Glasgow G12 8QQ, Lanark, Scotland; 3.Inst Pasteur Montevideo, Grp Biomol Simulat, Montevideo 11400, Uruguay; 4.Imperial Coll London, Natl Heart & Lung Inst, Imperial Ctr Translat & Expt Med, London W12 0NN, England; 5.Univ Calif Davis, Dept Pharmacol, Davis, CA 95616 USA |
推荐引用方式 GB/T 7714 | Surdo, Nicoletta C.,Berrera, Marco,Koschinski, Andreas,et al. FRET biosensor uncovers cAMP nano-domains at beta-adrenergic targets that dictate precise tuning of cardiac contractility[J]. NATURE COMMUNICATIONS,2017,8. |
APA | Surdo, Nicoletta C..,Berrera, Marco.,Koschinski, Andreas.,Brescia, Marcella.,Machado, Matias R..,...&Zaccolo, Manuela.(2017).FRET biosensor uncovers cAMP nano-domains at beta-adrenergic targets that dictate precise tuning of cardiac contractility.NATURE COMMUNICATIONS,8. |
MLA | Surdo, Nicoletta C.,et al."FRET biosensor uncovers cAMP nano-domains at beta-adrenergic targets that dictate precise tuning of cardiac contractility".NATURE COMMUNICATIONS 8(2017). |
条目包含的文件 | 条目无相关文件。 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论