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DOI | 10.1038/s41467-017-02699-3 |
Profiling surface proteins on individual exosomes using a proximity barcoding assay | |
Wu, Di1,2,3; Yan, Junhong1; Shen, Xia4,5,6; Sun, Yu7,11; Thulin, Mans8,12,13; Cai, Yanling9; Wik, Lotta1; Shen, Qiujin1; Oelrich, Johan3; Qian, Xiaoyan2; Dubois, K. Louise10; Ronquist, K. Goran10; Nilsson, Mats2; Landegren, Ulf1; Kamali-Moghaddam, Masood1 | |
2019-08-26 | |
发表期刊 | NATURE COMMUNICATIONS |
ISSN | 2041-1723 |
出版年 | 2019 |
卷号 | 10 |
文章类型 | Article |
语种 | 英语 |
国家 | Sweden; Scotland; Peoples R China |
英文摘要 | Exosomes have been implicated in numerous biological processes, and they may serve as important disease markers. Surface proteins on exosomes carry information about their tissues of origin. Because of the heterogeneity of exosomes it is desirable to investigate them individually, but this has so far remained impractical. Here, we demonstrate a proximity-dependent barcoding assay to profile surface proteins of individual exosomes using antibody-DNA conjugates and next-generation sequencing. We first validate the method using artificial streptavidin-oligonucleotide complexes, followed by analysis of the variable composition of surface proteins on individual exosomes, derived from human body fluids or cell culture media. Exosomes from different sources are characterized by the presence of specific combinations of surface proteins and their abundance, allowing exosomes to be separately quantified in mixed samples to serve as markers for tissue-specific engagement in disease. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000482567200002 |
WOS关键词 | PROSTASOMES ; VESICLES ; CELLS |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/203622 |
专题 | 资源环境科学 |
作者单位 | 1.Uppsala Univ, Dept Immunol Genet & Pathol, Sci Life Lab, SE-75185 Uppsala, Sweden; 2.Stockholm Univ, Sci Life Lab, Dept Biochem & Biophys, SE-17165 Solna, Sweden; 3.Vesicode AB, Nobels Vag 16, SE-17165 Solna, Sweden; 4.Univ Edinburgh, Usher Inst Populat Hlth Sci & Informat, Ctr Global Hlth Res, Teviot Pl, Edinburgh EH8 9AG, Midlothian, Scotland; 5.Karolinska Inst, Dept Med Epidemiol & Biostat, Nobels Vag 12 A, SE-17177 Stockholm, Sweden; 6.Sun Yat Sen Univ, Sch Life Sci, State Key Lab Biocontrol, Biostat Grp, CN-510000 Guangzhou, Guangdong, Peoples R China; 7.Uppsala Univ, Dept Mol Evolut, SE-75124 Uppsala, Sweden; 8.Uppsala Univ, Dept Stat, SE-75120 Uppsala, Sweden; 9.Second Peoples Hosp Shenzhen, Inst Translat Med, CN-518000 Shenzhen, Peoples R China; 10.Uppsala Univ, Dept Med Sci, Clin Chem, SE-75185 Uppsala, Sweden; 11.South China Agr Univ, Coll Life Sci, Guangdong Prov Key Lab Prot Funct & Regulat Agr O, Guangzhou 510642, Guangdong, Peoples R China; 12.Univ Edinburgh, Sch Math, Teviot Pl, Edinburgh EH8 9AG, Midlothian, Scotland; 13.Univ Edinburgh, Maxwell Inst Math Sci, Teviot Pl, Edinburgh EH8 9AG, Midlothian, Scotland |
推荐引用方式 GB/T 7714 | Wu, Di,Yan, Junhong,Shen, Xia,et al. Profiling surface proteins on individual exosomes using a proximity barcoding assay[J]. NATURE COMMUNICATIONS,2019,10. |
APA | Wu, Di.,Yan, Junhong.,Shen, Xia.,Sun, Yu.,Thulin, Mans.,...&Kamali-Moghaddam, Masood.(2019).Profiling surface proteins on individual exosomes using a proximity barcoding assay.NATURE COMMUNICATIONS,10. |
MLA | Wu, Di,et al."Profiling surface proteins on individual exosomes using a proximity barcoding assay".NATURE COMMUNICATIONS 10(2019). |
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