Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1038/s41467-017-02450-y |
Purification of cross-linked RNA-protein complexes by phenol-toluol extraction | |
Urdaneta, Erika C.1; Vieira-Vieira, Carlos H.2; Hick, Timon1; Wessels, Hans-Herrmann3,4; Figini, Davide1; Moschall, Rebecca5; Medenbach, Jan5; Ohler, Uwe3,4; Granneman, Sander6; Selbach, Matthias2; Beckmann, Benedikt M.1 | |
2019-03-01 | |
发表期刊 | NATURE COMMUNICATIONS
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ISSN | 2041-1723 |
出版年 | 2019 |
卷号 | 10 |
文章类型 | Article |
语种 | 英语 |
国家 | Germany; Scotland |
英文摘要 | Recent methodological advances allowed the identification of an increasing number of RNA-binding proteins (RBPs) and their RNA-binding sites. Most of those methods rely, however, on capturing proteins associated to polyadenylated RNAs which neglects RBPs bound to non-adenylate RNA classes (tRNA, rRNA, pre-mRNA) as well as the vast majority of species that lack poly-A tails in their mRNAs (including all archea and bacteria). We have developed the Phenol Toluol extraction (PTex) protocol that does not rely on a specific RNA sequence or motif for isolation of cross-linked ribonucleoproteins (RNPs), but rather purifies them based entirely on their physicochemical properties. PTex captures RBPs that bind to RNA as short as 30 nt, RNPs directly from animal tissue and can be used to simplify complex workflows such as PAR-CLIP. Finally, we provide a global RNA-bound proteome of human HEK293 cells and the bacterium Salmonella Typhimurium. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000459988600002 |
WOS关键词 | BINDING PROTEIN ; MESSENGER-RNA ; FUNCTIONAL-ANALYSIS ; EXOSOME ; LINKING ; CLIP ; IDENTIFICATION ; REVEALS ; SITES ; DNA |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/203604 |
专题 | 资源环境科学 |
作者单位 | 1.Humboldt Univ, IRI Life Sci, Philippstr 13, D-10115 Berlin, Germany; 2.Max Delbruck Ctr Mol Med, Robert Rossle Str 10, D-13125 Berlin, Germany; 3.Max Delbruck Ctr Mol Med, Berlin Inst Med Syst Biol, Robert Rossle Str 10, D-13125 Berlin, Germany; 4.Humboldt Univ, Dept Biol, Philippstr 13, D-10115 Berlin, Germany; 5.Univ Regensburg, Biochem 1, Univ Str 31, D-93053 Regensburg, Germany; 6.Univ Edinburgh, Ctr Syst & Synthet Biol SynthSys, Edinburgh EH9 3BF, Midlothian, Scotland |
推荐引用方式 GB/T 7714 | Urdaneta, Erika C.,Vieira-Vieira, Carlos H.,Hick, Timon,et al. Purification of cross-linked RNA-protein complexes by phenol-toluol extraction[J]. NATURE COMMUNICATIONS,2019,10. |
APA | Urdaneta, Erika C..,Vieira-Vieira, Carlos H..,Hick, Timon.,Wessels, Hans-Herrmann.,Figini, Davide.,...&Beckmann, Benedikt M..(2019).Purification of cross-linked RNA-protein complexes by phenol-toluol extraction.NATURE COMMUNICATIONS,10. |
MLA | Urdaneta, Erika C.,et al."Purification of cross-linked RNA-protein complexes by phenol-toluol extraction".NATURE COMMUNICATIONS 10(2019). |
条目包含的文件 | 条目无相关文件。 |
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