Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1038/s41467-017-02254-0 |
Allosteric activation of an ion channel triggered by modification of mechanosensitive nano-pockets | |
Kapsalis, Charalampos1; Wang, Bolin1,2,3; El Mkami, Hassane4,5; Pitt, Samantha J.6; Schnell, Jason R.7; Smith, Terry K.8; Lippiat, Jonathan D.3; Bode, Bela E.5; Pliotas, Christos1,2,3,5 | |
2019-10-10 | |
发表期刊 | NATURE COMMUNICATIONS |
ISSN | 2041-1723 |
出版年 | 2019 |
卷号 | 10 |
文章类型 | Article |
语种 | 英语 |
国家 | Scotland; England |
英文摘要 | Lipid availability within transmembrane nano-pockets of ion channels is linked with mechanosensation. However, the effect of hindering lipid-chain penetration into nano-pockets on channel structure has not been demonstrated. Here we identify nano-pockets on the large conductance mechanosensitive channel MscL, the high-pressure threshold channel. We restrict lipid-chain access to the nano-pockets by mutagenesis and sulfhydryl modification, and monitor channel conformation by PELDOR/DEER spectroscopy. For a single site located at the entrance of the nano-pockets and distal to the channel pore we generate an allosteric response in the absence of tension. Single-channel recordings reveal a significant decrease in the pressure activation threshold of the modified channel and a sub-conducting state in the absence of applied tension. Threshold is restored to wild-type levels upon reduction of the sulfhydryl modification. The modification associated with the conformational change restricts lipid access to the nano-pocket, interrupting the contact between the membrane and the channel that mediates mechanosensitivity. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000489558100002 |
WOS关键词 | ESCHERICHIA-COLI ; SMALL-CONDUCTANCE ; MYCOBACTERIUM-TUBERCULOSIS ; MEMBRANE-PROTEINS ; MSCL ; LIPIDS ; MECHANISM ; BILAYER ; PELDOR ; TRANSDUCTION |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/203589 |
专题 | 资源环境科学 |
作者单位 | 1.Univ St Andrews, Sch Biol, Biomed Sci Res Complex, St Andrews KY16 9ST, Fife, Scotland; 2.Univ Leeds, Astbury Ctr Struct Mol Biol, Leeds LS2 9JT, W Yorkshire, England; 3.Univ Leeds, Fac Biol Sci, Sch Biomed Sci, Leeds LS2 9JT, W Yorkshire, England; 4.Univ St Andrews, Sch Phys & Astron, St Andrews KY16 9SS, Fife, Scotland; 5.Univ St Andrews, Ctr Magnet Resonance, St Andrews KY16 9ST, Fife, Scotland; 6.Univ St Andrews, Sch Med, St Andrews KY16 9TF, Fife, Scotland; 7.Univ Oxford, Dept Biochem, Oxford OX1 3QU, England; 8.Univ St Andrews, Sch Chem, Biomed Sci Res Complex, St Andrews KY16 9ST, Fife, Scotland |
推荐引用方式 GB/T 7714 | Kapsalis, Charalampos,Wang, Bolin,El Mkami, Hassane,et al. Allosteric activation of an ion channel triggered by modification of mechanosensitive nano-pockets[J]. NATURE COMMUNICATIONS,2019,10. |
APA | Kapsalis, Charalampos.,Wang, Bolin.,El Mkami, Hassane.,Pitt, Samantha J..,Schnell, Jason R..,...&Pliotas, Christos.(2019).Allosteric activation of an ion channel triggered by modification of mechanosensitive nano-pockets.NATURE COMMUNICATIONS,10. |
MLA | Kapsalis, Charalampos,et al."Allosteric activation of an ion channel triggered by modification of mechanosensitive nano-pockets".NATURE COMMUNICATIONS 10(2019). |
条目包含的文件 | 条目无相关文件。 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论