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DOI | 10.1038/s41467-017-01577-2 |
Solid-state NMR spectroscopy based atomistic view of a membrane protein unfolding pathway | |
Xiao, Peng1,2; Bolton, David1,2; Munro, Rachel A.1,2; Brown, Leonid S.1,2; Ladizhansky, Vladimir1,2 | |
2019-08-27 | |
发表期刊 | NATURE COMMUNICATIONS |
ISSN | 2041-1723 |
出版年 | 2019 |
卷号 | 10 |
文章类型 | Article |
语种 | 英语 |
国家 | Canada |
英文摘要 | Membrane protein folding, structure, and function strongly depend on a cell membrane environment, yet detailed characterization of folding within a lipid bilayer is challenging. Studies of reversible unfolding yield valuable information on the energetics of folding and on the hierarchy of interactions contributing to protein stability. Here, we devise a methodology that combines hydrogen-deuterium (H/D) exchange and solid-state NMR (SSNMR) to follow membrane protein unfolding in lipid membranes at atomic resolution through detecting changes in the protein water-accessible surface, and concurrently monitoring the reversibility of unfolding. We obtain atomistic description of the reversible part of a thermally induced unfolding pathway of a seven-helical photoreceptor. The pathway is visualized through SSNMR-detected snapshots of H/D exchange patterns as a function of temperature, revealing the unfolding intermediate and its stabilizing factors. Our approach is transferable to other membrane proteins, and opens additional ways to characterize their unfolding and stabilizing interactions with atomic resolution. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000482711500001 |
WOS关键词 | ANABAENA SENSORY RHODOPSIN ; THERMODYNAMIC STABILITY ; SINGLE-MOLECULE ; BACTERIORHODOPSIN ; RESONANCE ; DYNAMICS ; SYSTEM ; SPINS ; N-15 |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/203531 |
专题 | 资源环境科学 |
作者单位 | 1.Univ Guelph, Dept Phys, 50 Stone Rd E, Guelph, ON N1G 2W1, Canada; 2.Univ Guelph, Biophys Interdept Grp, 50 Stone Rd E, Guelph, ON N1G 2W1, Canada |
推荐引用方式 GB/T 7714 | Xiao, Peng,Bolton, David,Munro, Rachel A.,et al. Solid-state NMR spectroscopy based atomistic view of a membrane protein unfolding pathway[J]. NATURE COMMUNICATIONS,2019,10. |
APA | Xiao, Peng,Bolton, David,Munro, Rachel A.,Brown, Leonid S.,&Ladizhansky, Vladimir.(2019).Solid-state NMR spectroscopy based atomistic view of a membrane protein unfolding pathway.NATURE COMMUNICATIONS,10. |
MLA | Xiao, Peng,et al."Solid-state NMR spectroscopy based atomistic view of a membrane protein unfolding pathway".NATURE COMMUNICATIONS 10(2019). |
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