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DOI | 10.1038/s41467-017-01606-0 |
Systematic identification of metabolites controlling gene expression in E. coli | |
Lempp, Martin1; Farke, Niklas1; Kuntz, Michelle1; Freibert, Sven Andreas2; Lill, Roland2,3; Link, Hannes1,3 | |
2019-10-02 | |
发表期刊 | NATURE COMMUNICATIONS |
ISSN | 2041-1723 |
出版年 | 2019 |
卷号 | 10 |
文章类型 | Article |
语种 | 英语 |
国家 | Germany |
英文摘要 | Metabolism controls gene expression through allosteric interactions between metabolites and transcription factors. These interactions are usually measured with in vitro assays, but there are no methods to identify them at a genome-scale in vivo. Here we show that dynamic transcriptome and metabolome data identify metabolites that control transcription factors in E. coli. By switching an E. coli culture between starvation and growth, we induce strong metabolite concentration changes and gene expression changes. Using Network Component Analysis we calculate the activities of 209 transcriptional regulators and correlate them with metabolites. This approach captures, for instance, the in vivo kinetics of CRP regulation by cyclic-AMP. By testing correlations between all pairs of transcription factors and metabolites, we predict putative effectors of 71 transcription factors, and validate five interactions in vitro. These results show that combining transcriptomics and metabolomics generates hypotheses about metabolism-transcription interactions that drive transitions between physiological states. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000488485100001 |
WOS关键词 | ESCHERICHIA-COLI ; REGULATORY NETWORKS ; OXIDATIVE STRESS ; PROTEIN ; RECONSTRUCTION ; STARVATION ; CAMP |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/203520 |
专题 | 资源环境科学 |
作者单位 | 1.Max Planck Inst Terr Microbiol, D-35043 Marburg, Germany; 2.Philipps Univ Marburg, Inst Zytobiol & Zytopathol, D-35033 Marburg, Germany; 3.Philipps Univ Marburg, LOEWE Zentrum Synthet Mikrobiol SYNMIKRO, D-35032 Marburg, Germany |
推荐引用方式 GB/T 7714 | Lempp, Martin,Farke, Niklas,Kuntz, Michelle,et al. Systematic identification of metabolites controlling gene expression in E. coli[J]. NATURE COMMUNICATIONS,2019,10. |
APA | Lempp, Martin,Farke, Niklas,Kuntz, Michelle,Freibert, Sven Andreas,Lill, Roland,&Link, Hannes.(2019).Systematic identification of metabolites controlling gene expression in E. coli.NATURE COMMUNICATIONS,10. |
MLA | Lempp, Martin,et al."Systematic identification of metabolites controlling gene expression in E. coli".NATURE COMMUNICATIONS 10(2019). |
条目包含的文件 | 条目无相关文件。 |
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