Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1038/s41467-017-01546-9 |
A fast and specific fluorescent probe for thioredoxin reductase that works via disulphide bond cleavage | |
Li, Xinming1,2; Zhang, Baoxin1,2; Yan, Chaoxian1,2; Li, Jin1,2; Wang, Song1,2; Wei, Xiangxu1,2; Jiang, Xiaoyan1,2; Zhou, Panpan1,2; Fang, Jianguo1,2 | |
2019-06-21 | |
发表期刊 | NATURE COMMUNICATIONS |
ISSN | 2041-1723 |
出版年 | 2019 |
卷号 | 10 |
文章类型 | Article |
语种 | 英语 |
国家 | Peoples R China |
英文摘要 | Small molecule probes are indispensable tools to explore diverse cellular events. However, finding a specific probe of a target remains a high challenge. Here we report the discovery of Fast-TRFS, a specific and superfast fluorogenic probe of mammalian thioredoxin reductase, a ubiquitous enzyme involved in regulation of diverse cellular redox signaling pathways. By systematically examining the processes of fluorophore release and reduction of cyclic disulfides/diselenides by the enzyme, structural factors that determine the response rate and specificity of the probe are disclosed. Mechanistic studies reveal that the fluorescence signal is switched on by a simple reduction of the disulfide bond within the probe, which is in stark contrast to the sensing mechanism of published probes. The favorable properties of Fast-TRFS enable development of a high-throughput screening assay to discover inhibitors of thioredoxin reductase by using crude tissue extracts as a source of the enzyme. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000472480500012 |
WOS关键词 | STRESS-MEDIATED APOPTOSIS ; MAMMALIAN THIOREDOXIN ; SECIS ELEMENTS ; SELENOCYSTEINE ; ANTIOXIDANT ; INHIBITION ; SELENIUM ; DISCOVERY ; ANALOGS ; SULFUR |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/203511 |
专题 | 资源环境科学 |
作者单位 | 1.Lanzhou Univ, State Key Lab Appl Organ Chem, Lanzhou 730000, Gansu, Peoples R China; 2.Lanzhou Univ, Coll Chem & Chem Engn, Lanzhou 730000, Gansu, Peoples R China |
推荐引用方式 GB/T 7714 | Li, Xinming,Zhang, Baoxin,Yan, Chaoxian,et al. A fast and specific fluorescent probe for thioredoxin reductase that works via disulphide bond cleavage[J]. NATURE COMMUNICATIONS,2019,10. |
APA | Li, Xinming.,Zhang, Baoxin.,Yan, Chaoxian.,Li, Jin.,Wang, Song.,...&Fang, Jianguo.(2019).A fast and specific fluorescent probe for thioredoxin reductase that works via disulphide bond cleavage.NATURE COMMUNICATIONS,10. |
MLA | Li, Xinming,et al."A fast and specific fluorescent probe for thioredoxin reductase that works via disulphide bond cleavage".NATURE COMMUNICATIONS 10(2019). |
条目包含的文件 | 条目无相关文件。 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论