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DOI | 10.1038/s41467-017-00855-3 |
A conserved dimer interface connects ERH and YTH family proteins to promote gene silencing | |
Xie, Guodong1; Vo, Tommy V.2; Thillainadesan, Gobi2; Holla, Sahana2; Zhang, Beibei1; Jiang, Yiyang1; Lv, Mengqi1; Xu, Zheng1; Wang, Chongyuan1; Balachandran, Vanivilasini2; Shi, Yunyu1; Li, Fudong1; Grewal, Shiv I. S.2 | |
2019-01-16 | |
发表期刊 | NATURE COMMUNICATIONS
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ISSN | 2041-1723 |
出版年 | 2019 |
卷号 | 10 |
文章类型 | Article |
语种 | 英语 |
国家 | Peoples R China; USA |
英文摘要 | Gene regulatory mechanisms rely on a complex network of RNA processing factors to prevent untimely gene expression. In fission yeast, the highly conserved ortholog of human ERH, called Erh1, interacts with the YTH family RNA binding protein Mmi1 to form the Erh1-Mmi1 complex (EMC) implicated in gametogenic gene silencing. However, the structural basis of EMC assembly and its functions are poorly understood. Here, we present the co-crystal structure of the EMC that consists of Erh1 homodimers interacting with Mmi1 in a 2: 2 stoichiometry via a conserved molecular interface. Structure-guided mutation of the Mmi1(Trp112) residue, which is required for Erh1 binding, causes defects in facultative heterochromatin assembly and gene silencing while leaving Mmi1-mediated transcription termination intact. Indeed, EMC targets masked in mmi1 Delta due to termination defects are revealed in mmi1(W112A). Our study delineates EMC requirements in gene silencing and identifies an ERH interface required for interaction with an RNA binding protein. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000455762200013 |
WOS关键词 | RNA-PROCESSING FACTORS ; SELECTIVE ELIMINATION ; HETEROCHROMATIN FORMATION ; MESSENGER-RNAS ; EXPRESSION ; TRANSCRIPTION ; MEIOSIS ; DOMAIN ; MACHINERY ; ENHANCER |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/203467 |
专题 | 资源环境科学 |
作者单位 | 1.Univ Sci & Technol China, Sch Life Sci, Hefei Natl Lab Phys Sci Microscale, Hefei 230026, Anhui, Peoples R China; 2.NCI, Lab Biochem & Mol Biol, NIH, Bethesda, MD 20892 USA |
推荐引用方式 GB/T 7714 | Xie, Guodong,Vo, Tommy V.,Thillainadesan, Gobi,et al. A conserved dimer interface connects ERH and YTH family proteins to promote gene silencing[J]. NATURE COMMUNICATIONS,2019,10. |
APA | Xie, Guodong.,Vo, Tommy V..,Thillainadesan, Gobi.,Holla, Sahana.,Zhang, Beibei.,...&Grewal, Shiv I. S..(2019).A conserved dimer interface connects ERH and YTH family proteins to promote gene silencing.NATURE COMMUNICATIONS,10. |
MLA | Xie, Guodong,et al."A conserved dimer interface connects ERH and YTH family proteins to promote gene silencing".NATURE COMMUNICATIONS 10(2019). |
条目包含的文件 | 条目无相关文件。 |
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