Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1038/s41467-017-01086-2 |
Experimental and computational analyses reveal that environmental restrictions shape HIV-1 spread in 3D cultures | |
Imle, Andrea1,10; Kumberger, Peter2; Schnellbaecher, Nikolas D.3,4; Fehr, Jana2,11; Carrillo-Bustamante, Paola2,12; Ales, Janez5; Schmidt, Philip5; Ritter, Christian6,7; Godinez, William J.6,7; Mueller, Barbara8; Rohr, Karl6,7; Hamprecht, Fred A.5; Schwarz, Ulrich S.3,4; Graw, Frederik2; Fackler, Oliver T.1,9 | |
2019-05-13 | |
发表期刊 | NATURE COMMUNICATIONS |
ISSN | 2041-1723 |
出版年 | 2019 |
卷号 | 10 |
文章类型 | Article |
语种 | 英语 |
国家 | Germany |
英文摘要 | Pathogens face varying microenvironments in vivo, but suitable experimental systems and analysis tools to dissect how three-dimensional (3D) tissue environments impact pathogen spread are lacking. Here we develop an Integrative method to Study Pathogen spread by Experiment and Computation within Tissue-like 3D cultures (INSPECT-3D), combining quantification of pathogen replication with imaging to study single-cell and cell population dynamics. We apply INSPECT-3D to analyze HIV-1 spread between primary human CD4 T-lymphocytes using collagen as tissue-like 3D-scaffold. Measurements of virus replication, infectivity, diffusion, cellular motility and interactions are combined by mathematical analyses into an integrated spatial infection model to estimate parameters governing HIV-1 spread. This reveals that environmental restrictions limit infection by cell-free virions but promote cell-associated HIV-1 transmission. Experimental validation identifies cell motility and density as essential determinants of efficacy and mode of HIV-1 spread in 3D. INSPECT-3D represents an adaptable method for quantitative time-resolved analyses of 3D pathogen spread. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000467704100003 |
WOS关键词 | CELL-TO-CELL ; IMMUNODEFICIENCY-VIRUS TYPE-1 ; SINGLE-PARTICLE TRACKING ; ANTIGEN PRESENTATION ; VIROLOGICAL SYNAPSE ; NEF INTERFERES ; MIGRATION ; LYMPHOCYTE ; INFECTION ; MATRIX |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/203459 |
专题 | 资源环境科学 |
作者单位 | 1.Univ Hosp Heidelberg, Ctr Integrat Infect Dis Res, Dept Infect Dis, Integrat Virol, D-69120 Heidelberg, Germany; 2.Heidelberg Univ, Ctr Modelling & Simulat Biosci, BioQuant, D-69120 Heidelberg, Germany; 3.Heidelberg Univ, Inst Theoret Phys, D-69120 Heidelberg, Germany; 4.Heidelberg Univ, BioQuant, D-69120 Heidelberg, Germany; 5.Heidelberg Univ, HCI IWR, D-69120 Heidelberg, Germany; 6.Heidelberg Univ, Biomed Comp Vis Grp, BioQuant, IPMB, D-69120 Heidelberg, Germany; 7.Heidelberg Univ, DKFZ, D-69120 Heidelberg, Germany; 8.Univ Hosp Heidelberg, Ctr Integrat Infect Dis Res, Dept Infect Dis, D-69120 Heidelberg, Germany; 9.German Ctr Infect Res DZIF, Partner Site Heidelberg, Heidelberg, Germany; 10.European Mol Biol Lab, Cell Biol & Biophys Unit, D-69117 Heidelberg, Germany; 11.Hasso Plattner Inst, Digital Hlth & Machine Learning, D-14482 Potsdam, Germany; 12.Max Planck Inst Infect Biol, Vector Biol Unit, D-10117 Berlin, Germany |
推荐引用方式 GB/T 7714 | Imle, Andrea,Kumberger, Peter,Schnellbaecher, Nikolas D.,et al. Experimental and computational analyses reveal that environmental restrictions shape HIV-1 spread in 3D cultures[J]. NATURE COMMUNICATIONS,2019,10. |
APA | Imle, Andrea.,Kumberger, Peter.,Schnellbaecher, Nikolas D..,Fehr, Jana.,Carrillo-Bustamante, Paola.,...&Fackler, Oliver T..(2019).Experimental and computational analyses reveal that environmental restrictions shape HIV-1 spread in 3D cultures.NATURE COMMUNICATIONS,10. |
MLA | Imle, Andrea,et al."Experimental and computational analyses reveal that environmental restrictions shape HIV-1 spread in 3D cultures".NATURE COMMUNICATIONS 10(2019). |
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