Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1038/s41467-017-00744-9 |
A comprehensive single cell transcriptional landscape of human hematopoietic progenitors | |
Pellin, Danilo1; Loperfido, Mariana1; Baricordi, Cristina1; Wolock, Samuel L.2; Montepeloso, Annita1; Weinberg, Olga K.3,4; Biffi, Alessandra1; Klein, Allon M.2; Biasco, Luca1,5 | |
2019-06-03 | |
发表期刊 | NATURE COMMUNICATIONS
![]() |
ISSN | 2041-1723 |
出版年 | 2019 |
卷号 | 10 |
文章类型 | Article |
语种 | 英语 |
国家 | USA; England |
英文摘要 | Hematopoietic Stem/Progenitor cells (HSPCs) are endowed with the role of maintaining a diverse pool of blood cells throughout the human life. Despite recent efforts, the nature of the early cell fate decisions remains contentious. Using single-cell RNA-Seq, we show that existing approaches to stratify bone marrow CD34+ cells reveal a hierarchically-structured transcriptional landscape of hematopoietic differentiation. Still, this landscape misses important early fate decisions. We here provide a broader transcriptional profiling of bone marrow lineage negative hematopoietic progenitors that recovers a key missing branchpoint into basophils and expands our understanding of the underlying structure of early adult human haematopoiesis. We also show that this map has strong similarities in topology and gene expression to that found in mouse. Finally, we identify the sialomucin CD164, as a reliable marker for the earliest branches of HSPCs specification and we showed how its use can foster the design of alternative transplantation cell products. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000469909200013 |
WOS关键词 | STEM-CELLS ; DIFFUSION MAPS ; CD164 ; SIALOMUCIN ; DYNAMICS ; PATTERNS ; REVEALS ; CD34(+) ; MOUSE |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/203446 |
专题 | 资源环境科学 |
作者单位 | 1.Harvard Med Sch, Gene Therapy Program, Dana Farber Boston Childrens Canc & Blood Disorde, Boston, MA 02115 USA; 2.Harvard Med Sch, Dept Syst Biol, Boston, MA 02115 USA; 3.Boston Childrens Hosp, Dept Pathol, Boston, MA 02115 USA; 4.Harvard Med Sch, Boston, MA 02115 USA; 5.UCL, Great Ormond St Inst Child Hlth, Fac Populat Hlth Sci, London WC1N 1EH, England |
推荐引用方式 GB/T 7714 | Pellin, Danilo,Loperfido, Mariana,Baricordi, Cristina,et al. A comprehensive single cell transcriptional landscape of human hematopoietic progenitors[J]. NATURE COMMUNICATIONS,2019,10. |
APA | Pellin, Danilo.,Loperfido, Mariana.,Baricordi, Cristina.,Wolock, Samuel L..,Montepeloso, Annita.,...&Biasco, Luca.(2019).A comprehensive single cell transcriptional landscape of human hematopoietic progenitors.NATURE COMMUNICATIONS,10. |
MLA | Pellin, Danilo,et al."A comprehensive single cell transcriptional landscape of human hematopoietic progenitors".NATURE COMMUNICATIONS 10(2019). |
条目包含的文件 | 条目无相关文件。 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论