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DOI | 10.1038/s41467-017-00820-0 |
Human induced pluripotent stem cell-derived vocal fold mucosa mimics development and responses to smoke exposure | |
Lungova, Vlasta1; Chen, Xia1; Wang, Ziyue2; Kendziorski, Christina2; Thibeault, Susan L.1 | |
2019-09-24 | |
发表期刊 | NATURE COMMUNICATIONS |
ISSN | 2041-1723 |
出版年 | 2019 |
卷号 | 10 |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | Development of treatments for vocal dysphonia has been inhibited by lack of human vocal fold (VF) mucosa models because of difficulty in procuring VF epithelial cells, epithelial cells' limited proliferative capacity and absence of cell lines. Here we report development of engineered VF mucosae from hiPSC, transfected via TALEN constructs for green fluorescent protein, that mimic development of VF epithelial cells in utero. Modulation of FGF signaling achieves stratified squamous epithelium from definitive and anterior foregut derived cultures. Robust culturing of these cells on collagen-fibroblast constructs produces three-dimensional models comparable to in vivo VF mucosa. Furthermore, we demonstrate mucosal inflammation upon exposure of these constructs to 5% cigarette smoke extract. Upregulation of pro-inflammatory genes in epithelium and fibroblasts leads to aberrant VF mucosa remodeling. Collectively, our results demonstrate that hiPSC-derived VF mucosa is a versatile tool for future investigation of genetic and molecular mechanisms underlying epithelium-fibroblasts interactions in health and disease. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000487356700001 |
WOS关键词 | LESIONS ; MODEL ; DIFFERENTIATION ; DISORDERS ; DYSPLASIA ; NODULES ; IMPACT |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/203442 |
专题 | 资源环境科学 |
作者单位 | 1.Univ Wisconsin, Dept Surg, Wisconsin Inst Med Res, Madison, WI 53706 USA; 2.Univ Wisconsin, Dept Biostat & Med Informat, Madison, WI USA |
推荐引用方式 GB/T 7714 | Lungova, Vlasta,Chen, Xia,Wang, Ziyue,et al. Human induced pluripotent stem cell-derived vocal fold mucosa mimics development and responses to smoke exposure[J]. NATURE COMMUNICATIONS,2019,10. |
APA | Lungova, Vlasta,Chen, Xia,Wang, Ziyue,Kendziorski, Christina,&Thibeault, Susan L..(2019).Human induced pluripotent stem cell-derived vocal fold mucosa mimics development and responses to smoke exposure.NATURE COMMUNICATIONS,10. |
MLA | Lungova, Vlasta,et al."Human induced pluripotent stem cell-derived vocal fold mucosa mimics development and responses to smoke exposure".NATURE COMMUNICATIONS 10(2019). |
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