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DOI | 10.1038/s41467-017-00529-0 |
Chronic Chlamydia infection in human organoids increases stemness and promotes age-dependent CpG methylation | |
Kessler, Mirjana1; Hoffmann, Karen1; Fritsche, Kristin1; Brinkmann, Volker1; Mollenkopf, Hans-Joachim1; Thieck, Oliver1; da Costa, Ana Rita Teixeira1; Braicu, Elena, I2; Sehouli, Jalid2; Mangler, Mandy3; Berger, Hilmar1; Meyer, Thomas F.1 | |
2019-03-18 | |
发表期刊 | NATURE COMMUNICATIONS |
ISSN | 2041-1723 |
出版年 | 2019 |
卷号 | 10 |
文章类型 | Article |
语种 | 英语 |
国家 | Germany |
英文摘要 | Chronic infections of the fallopian tubes with Chlamydia trachomatis (Ctr) cause scarring and can lead to infertility. Here we use human fallopian tube organoids and genital Ctr serovars D, K and E for long-term in vitro analysis. The epithelial monolayer responds with active expulsion of the bacteria into the lumen and with compensatory cellular proliferation-demonstrating a role of epithelial homeostasis in the defense against this pathogen. In addition, Ctr infection activates LIF signaling, which we find to be an essential regulator of stemness in the organoids. Infected organoids exhibit a less differentiated phenotype with higher stemness potential, as confirmed by increased organoid forming efficiency. Moreover, Ctr increases hypermethylation of DNA, which is an indicator of accelerated molecular aging. Thus, the chronic organoid infection model suggests that Ctr has a long-term impact on the epithelium. These heritable changes might be a contributing factor in the development of tubal pathologies, including the initiation of high grade serous ovarian cancer. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000461413800001 |
WOS关键词 | LEUKEMIA-INHIBITORY FACTOR ; OVARIAN-CANCER ; TRACHOMATIS INFECTION ; EPIGENETIC REGULATION ; CELLS ; DIFFERENTIATION ; ACTIVATION ; PROTEINS ; POLYCOMB ; REVEALS |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/203422 |
专题 | 资源环境科学 |
作者单位 | 1.Max Planck Inst Infect Biol, Dept Mol Biol, Charitepl 1, D-10117 Berlin, Germany; 2.Charite, Dept Gynecol, Campus Virchow, Augustenburger Pl 1, D-13353 Berlin, Germany; 3.Auguste Viktoria Klinikum, Dept Gynecol & Obstet, Rubensstr 125, D-12157 Berlin, Germany |
推荐引用方式 GB/T 7714 | Kessler, Mirjana,Hoffmann, Karen,Fritsche, Kristin,et al. Chronic Chlamydia infection in human organoids increases stemness and promotes age-dependent CpG methylation[J]. NATURE COMMUNICATIONS,2019,10. |
APA | Kessler, Mirjana.,Hoffmann, Karen.,Fritsche, Kristin.,Brinkmann, Volker.,Mollenkopf, Hans-Joachim.,...&Meyer, Thomas F..(2019).Chronic Chlamydia infection in human organoids increases stemness and promotes age-dependent CpG methylation.NATURE COMMUNICATIONS,10. |
MLA | Kessler, Mirjana,et al."Chronic Chlamydia infection in human organoids increases stemness and promotes age-dependent CpG methylation".NATURE COMMUNICATIONS 10(2019). |
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