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DOI | 10.1038/s41467-017-00219-x |
A cell-free platform for the prenylation of natural products and application to cannabinoid production | |
Valliere, Meaghan A.1; Korman, Tyler P.1; Woodall, Nicholas B.2; Khitrov, Gregory A.1; Taylor, Robert E.1; Baker, David2; Bowie, James U.1 | |
2019-02-04 | |
发表期刊 | NATURE COMMUNICATIONS
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ISSN | 2041-1723 |
出版年 | 2019 |
卷号 | 10 |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | Prenylation of natural compounds adds structural diversity, alters biological activity, and enhances therapeutic potential. Because prenylated compounds often have a low natural abundance, alternative production methods are needed. Metabolic engineering enables natural product biosynthesis from inexpensive biomass, but is limited by the complexity of secondary metabolite pathways, intermediate and product toxicities, and substrate accessibility. Alternatively, enzyme catalyzed prenyl transfer provides excellent regio- and stereo-specificity, but requires expensive isoprenyl pyrophosphate substrates. Here we develop a flexible cell-free enzymatic prenylating system that generates isoprenyl pyropho-sphate substrates from glucose to prenylate an array of natural products. The system provides an efficient route to cannabinoid precursors cannabigerolic acid (CBGA) and cannabigerovarinic acid (CBGVA) at > 1 g/L, and a single enzymatic step converts the precursors into cannabidiolic acid (CBDA) and cannabidivarinic acid (CBDVA). Cell-free methods may provide a powerful alternative to metabolic engineering for chemicals that are hard to produce in living organisms. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000457582900009 |
WOS关键词 | CANNABIDIOLIC-ACID SYNTHASE ; CANNABIGEROLIC ACID ; SYNTHETIC PATHWAY ; SATIVA ; ENZYME ; BIOLOGY ; ROUTE |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/203400 |
专题 | 资源环境科学 |
作者单位 | 1.Univ Calif Los Angeles, UCLA DOE Inst, Mol Biol Inst, Dept Chem & Biochem, Los Angeles, CA 90095 USA; 2.Univ Washington, Inst Prot Design, Dept Biochem, Seattle, WA 98105 USA |
推荐引用方式 GB/T 7714 | Valliere, Meaghan A.,Korman, Tyler P.,Woodall, Nicholas B.,et al. A cell-free platform for the prenylation of natural products and application to cannabinoid production[J]. NATURE COMMUNICATIONS,2019,10. |
APA | Valliere, Meaghan A..,Korman, Tyler P..,Woodall, Nicholas B..,Khitrov, Gregory A..,Taylor, Robert E..,...&Bowie, James U..(2019).A cell-free platform for the prenylation of natural products and application to cannabinoid production.NATURE COMMUNICATIONS,10. |
MLA | Valliere, Meaghan A.,et al."A cell-free platform for the prenylation of natural products and application to cannabinoid production".NATURE COMMUNICATIONS 10(2019). |
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