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DOI | 10.1038/s41467-017-00075-9 |
Cryo-EM structures of the pore-forming A subunit from the Yersinia entomophaga ABC toxin | |
Piper, Sarah J.1,2; Brillault, Lou1,2; Rothnagel, Rosalba2; Croll, Tristan, I3; Box, Joseph K.1; Chassagnon, Irene1; Scherer, Sebastian4; Goldie, Kenneth N.4; Jones, Sandra A.5; Schepers, Femke6,7; Hartley-Tassell, Lauren8; Ve, Thomas8; Busby, Jason N.9; Dalziel, Julie E.7; Lott, J. Shaun9; Hankamer, Ben2; Stahlberg, Henning4; Hurst, Mark R. H.5; Landsberg, Michael J.1,2 | |
2019-04-26 | |
发表期刊 | NATURE COMMUNICATIONS |
ISSN | 2041-1723 |
出版年 | 2019 |
卷号 | 10 |
文章类型 | Article |
语种 | 英语 |
国家 | Australia; England; Switzerland; New Zealand; Netherlands |
英文摘要 | ABC toxins are pore-forming virulence factors produced by pathogenic bacteria. YenTcA is the pore-forming and membrane binding A subunit of the ABC toxin YenTc, produced by the insect pathogen Yersinia entomophaga. Here we present cryo-EM structures of YenTcA, purified from the native source. The soluble pre-pore structure, determined at an average resolution of 4.4 A, reveals a pentameric assembly that in contrast to other characterised ABC toxins is formed by two TcA-like proteins (YenA1 and YenA2) and decorated by two endochitinases (Chi1 and Chi2). We also identify conformational changes that accompany membrane pore formation by visualising YenTcA inserted into liposomes. A clear outward rotation of the Chi1 subunits allows for access of the protruding translocation pore to the membrane. Our results highlight structural and functional diversity within the ABC toxin subfamily, explaining how different ABC toxins are capable of recognising diverse hosts. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000465838600004 |
WOS关键词 | TC TOXIN ; COLEOPTERA SCARABAEIDAE ; COSTELYTRA-ZEALANDICA ; MEMBRANE INSERTION ; PROTEIN ; PH ; BINDING ; COMPLEX ; IMPLEMENTATION ; IDENTIFICATION |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/203394 |
专题 | 资源环境科学 |
作者单位 | 1.Univ Queensland, Sch Chem & Mol Biosci, St Lucia, Qld 4072, Australia; 2.Univ Queensland, Inst Mol Biosci, St Lucia, Qld 4072, Australia; 3.Univ Cambridge, Cambridge Inst Med Res, Cambridge CB2 0XY, Cambs, England; 4.Univ Basel, Ctr Cellular Imaging & NanoAnalyt, Biozentrum, CH-4058 Basel, Switzerland; 5.AgResearch, Forage Sci Grp, Christchurch 8140, New Zealand; 6.Leiden Univ, Fac Sci, NL-2300 RA Leiden, Netherlands; 7.AgResearch, Food & Biobased Prod Grp, Palmerston North 4442, New Zealand; 8.Griffith Univ, Inst Glyc, Gold Coast, Qld 4222, Australia; 9.Univ Auckland, Sch Biol Sci, Auckland 1142, New Zealand |
推荐引用方式 GB/T 7714 | Piper, Sarah J.,Brillault, Lou,Rothnagel, Rosalba,et al. Cryo-EM structures of the pore-forming A subunit from the Yersinia entomophaga ABC toxin[J]. NATURE COMMUNICATIONS,2019,10. |
APA | Piper, Sarah J..,Brillault, Lou.,Rothnagel, Rosalba.,Croll, Tristan, I.,Box, Joseph K..,...&Landsberg, Michael J..(2019).Cryo-EM structures of the pore-forming A subunit from the Yersinia entomophaga ABC toxin.NATURE COMMUNICATIONS,10. |
MLA | Piper, Sarah J.,et al."Cryo-EM structures of the pore-forming A subunit from the Yersinia entomophaga ABC toxin".NATURE COMMUNICATIONS 10(2019). |
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