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DOI | 10.1038/ncomms15386 |
Retroviral integration into nucleosomes through DNA looping and sliding along the histone octamer | |
Wilson, Marcus D.1,7; Renault, Ludovic1,8; Maskell, Daniel P.2,9; Ghoneim, Mohamed3,4; Pye, Valerie E.2; Nans, Andrea5; Rueda, David S.3,4; Cherepanov, Peter2,6; Costa, Alessandro1 | |
2019-09-13 | |
发表期刊 | NATURE COMMUNICATIONS |
ISSN | 2041-1723 |
出版年 | 2019 |
卷号 | 10 |
文章类型 | Article |
语种 | 英语 |
国家 | England; Scotland; Netherlands |
英文摘要 | Retroviral integrase can efficiently utilise nucleosomes for insertion of the reverse-transcribed viral DNA. In face of the structural constraints imposed by the nucleosomal structure, integrase gains access to the scissile phosphodiester bonds by lifting DNA off the histone octamer at the site of integration. To clarify the mechanism of DNA looping by integrase, we determined a 3.9 angstrom resolution structure of the prototype foamy virus intasome engaged with a nucleosome core particle. The structural data along with complementary single-molecule Forster resonance energy transfer measurements reveal twisting and sliding of the nucleosomal DNA arm proximal to the integration site. Sliding the nucleosomal DNA by approximately two base pairs along the histone octamer accommodates the necessary DNA lifting from the histone H2A-H2B subunits to allow engagement with the intasome. Thus, retroviral integration into nucleosomes involves the looping-and-sliding mechanism for nucleosomal DNA repositioning, bearing unexpected similarities to chromatin remodelers. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000485685900028 |
WOS关键词 | CRYO-EM ; CORE PARTICLE ; CRYSTAL-STRUCTURE ; STRUCTURAL BASIS ; CRYOELECTRON MICROSCOPY ; NUCLEIC-ACID ; RECOGNITION ; BINDING ; VISUALIZATION ; COMPLEXES |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/203318 |
专题 | 资源环境科学 |
作者单位 | 1.Francis Crick Inst, Macromol Machines Lab, London NW1 1AT, England; 2.Francis Crick Inst, Chromatin Struct & Mobile DNA Lab, London NW1 1AT, England; 3.London Inst Med Sci, MRC, Single Mol Imaging Grp, London W12 0NN, England; 4.Imperial Coll London, Dept Med, Mol Virol, London W12 0NN, England; 5.Francis Crick Inst, Struct Biol Sci Technol Platform, London NW1 1AT, England; 6.Imperial Coll London, Dept Med, St Marys Campus,Norfolk Pl, London W2 1PG, England; 7.Univ Edinburgh, Wellcome Ctr Cell Biol, Edinburgh EH9 3JR, Midlothian, Scotland; 8.Leiden Univ, NeCEN, NL-2333 CC Leiden, Netherlands; 9.Fac Biol Sci, Leeds LS2 9JT, W Yorkshire, England |
推荐引用方式 GB/T 7714 | Wilson, Marcus D.,Renault, Ludovic,Maskell, Daniel P.,et al. Retroviral integration into nucleosomes through DNA looping and sliding along the histone octamer[J]. NATURE COMMUNICATIONS,2019,10. |
APA | Wilson, Marcus D..,Renault, Ludovic.,Maskell, Daniel P..,Ghoneim, Mohamed.,Pye, Valerie E..,...&Costa, Alessandro.(2019).Retroviral integration into nucleosomes through DNA looping and sliding along the histone octamer.NATURE COMMUNICATIONS,10. |
MLA | Wilson, Marcus D.,et al."Retroviral integration into nucleosomes through DNA looping and sliding along the histone octamer".NATURE COMMUNICATIONS 10(2019). |
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