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| DOI | 10.1038/ncomms14966 |
| Controlling CRISPR-Cas9 with ligand-activated and ligand-deactivated sgRNAs | |
| Kundert, Kale1; Lucas, James E.2; Watters, Kyle E.3; Fellmann, Christof3; Ng, Andrew H.2; Heineike, Benjamin M.4; Fitzsimmons, Christina M.5,11; Oakes, Benjamin L.3; Qu, Jiuxin6; Prasad, Neha6; Rosenberg, Oren S.6,7; Savage, David F.3; El-Samad, Hana7,8; Doudna, Jennifer A.3,9; Kortemme, Tanja7,10 | |
| 2019-05-09 | |
| 发表期刊 | NATURE COMMUNICATIONS
 |
| ISSN | 2041-1723 |
| 出版年 | 2019 |
| 卷号 | 10 |
| 文章类型 | Article |
| 语种 | 英语 |
| 国家 | USA |
| 英文摘要 | The CRISPR-Cas9 system provides the ability to edit, repress, activate, or mark any gene (or DNA element) by pairing of a programmable single guide RNA (sgRNA) with a complementary sequence on the DNA target. Here we present a new method for small-molecule control of CRISPR-Cas9 function through insertion of RNA aptamers into the sgRNA. We show that CRISPR-Cas9-based gene repression (CRISPRi) can be either activated or deactivated in a dose-dependent fashion over a >10-fold dynamic range in response to two different small-molecule ligands. Since our system acts directly on each target-specific sgRNA, it enables new applications that require differential and opposing temporal control of multiple genes. |
| 领域 | 资源环境 |
| 收录类别 | SCI-E |
| WOS记录号 | WOS:000467535300003 |
| WOS关键词 | GENE-EXPRESSION ; GLOBAL ANALYSIS ; RNA APTAMERS ; HUMAN-CELLS ; GUIDE RNA ; CAS9 ; TRANSCRIPTION ; YEAST ; INTERROGATION ; ENDONUCLEASE |
| WOS类目 | Multidisciplinary Sciences |
| WOS研究方向 | Science & Technology - Other Topics |
| URL | 查看原文 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/203312 |
| 专题 | 资源环境科学 |
| 作者单位 | 1.Univ Calif San Francisco, Grad Grp Biophys, San Francisco, CA 94158 USA; 2.Univ Calif San Francisco, UC Berkeley UCSF Grad Program Bioengn, San Francisco, CA 94158 USA; 3.Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94704 USA; 4.Univ Calif San Francisco, Bioinformat Grad Program, San Francisco, CA 94158 USA; 5.Univ Calif San Francisco, Chem & Chem Biol Grad Program, San Francisco, CA 94158 USA; 6.Univ Calif San Francisco, Dept Med, San Francisco, CA 94158 USA; 7.Chan Zuckerberg Biohub, San Francisco, CA 94158 USA; 8.Univ Calif San Francisco, Dept Biochem & Biophys, San Francisco, CA 94158 USA; 9.Univ Calif Berkeley, Howard Hughes Med Inst, Berkeley, CA 94704 USA; 10.Univ Calif San Francisco, Dept Bioengn & Therapeut Sci, San Francisco, CA 94158 USA; 11.NCI, Cell Biol Lab, Ctr Canc Res, NIH, Bldg 37, Bethesda, MD 20892 USA |
| 推荐引用方式 GB/T 7714 | Kundert, Kale,Lucas, James E.,Watters, Kyle E.,et al. Controlling CRISPR-Cas9 with ligand-activated and ligand-deactivated sgRNAs[J]. NATURE COMMUNICATIONS,2019,10. |
| APA | Kundert, Kale.,Lucas, James E..,Watters, Kyle E..,Fellmann, Christof.,Ng, Andrew H..,...&Kortemme, Tanja.(2019).Controlling CRISPR-Cas9 with ligand-activated and ligand-deactivated sgRNAs.NATURE COMMUNICATIONS,10. |
| MLA | Kundert, Kale,et al."Controlling CRISPR-Cas9 with ligand-activated and ligand-deactivated sgRNAs".NATURE COMMUNICATIONS 10(2019). |
| 条目包含的文件 | 条目无相关文件。 | |||||
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