Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1038/ncomms15243 |
De novo synthesized Min proteins drive oscillatory liposome deformation and regulate FtsA-FtsZ cytoskeletal patterns | |
Godino, Elisa; Lopez, Jonas Noguera; Foschepoth, David; Cleij, Celine; Doerr, Anne; Castella, Clara Ferrer; Danelon, Christophe | |
2019-10-31 | |
发表期刊 | NATURE COMMUNICATIONS
![]() |
ISSN | 2041-1723 |
出版年 | 2019 |
卷号 | 10 |
文章类型 | Article |
语种 | 英语 |
国家 | Netherlands |
英文摘要 | The Min biochemical network regulates bacterial cell division and is a prototypical example of self-organizing molecular systems. Cell-free assays relying on purified proteins have shown that MinE and MinD self-organize into surface waves and oscillatory patterns. In the context of developing a synthetic cell from elementary biological modules, harnessing Min oscillations might allow us to implement higher-order cellular functions. To convey hereditary information, the Min system must be encoded in a DNA molecule that can be copied, transcribed, and translated. Here, the MinD and MinE proteins are synthesized de novo from their genes inside liposomes. Dynamic protein patterns and accompanying liposome shape deformation are observed. When integrated with the cytoskeletal proteins FtsA and FtsZ, the synthetic Min system is able to dynamically regulate FtsZ patterns. By enabling genetic control over Min protein self-organization and membrane remodeling, our methodology offers unique opportunities towards directed evolution of bacterial division processes in vitro. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000493438700017 |
WOS关键词 | TO-POLE OSCILLATION ; ESCHERICHIA-COLI ; MEMBRANE INTERACTION ; DIVISION INHIBITOR ; CELL ; LOCALIZATION ; EXPRESSION ; PLACEMENT ; DYNAMICS ; FISSION |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/203302 |
专题 | 资源环境科学 |
作者单位 | Delft Univ Technol, Kavli Inst Nanosci, Dept Bionanosci, Maasweg 9, NL-2629HZ Delft, Netherlands |
推荐引用方式 GB/T 7714 | Godino, Elisa,Lopez, Jonas Noguera,Foschepoth, David,et al. De novo synthesized Min proteins drive oscillatory liposome deformation and regulate FtsA-FtsZ cytoskeletal patterns[J]. NATURE COMMUNICATIONS,2019,10. |
APA | Godino, Elisa.,Lopez, Jonas Noguera.,Foschepoth, David.,Cleij, Celine.,Doerr, Anne.,...&Danelon, Christophe.(2019).De novo synthesized Min proteins drive oscillatory liposome deformation and regulate FtsA-FtsZ cytoskeletal patterns.NATURE COMMUNICATIONS,10. |
MLA | Godino, Elisa,et al."De novo synthesized Min proteins drive oscillatory liposome deformation and regulate FtsA-FtsZ cytoskeletal patterns".NATURE COMMUNICATIONS 10(2019). |
条目包含的文件 | 条目无相关文件。 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论