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DOI | 10.1038/ncomms15361 |
Regulatory mechanisms underlying coordination of amino acid and glucose catabolism in Escherichia coli | |
Zampieri, Mattia; Hoerl, Manuel; Hotz, Florian; Mueller, Nicola F.; Sauer, Uwe | |
2019-07-26 | |
发表期刊 | NATURE COMMUNICATIONS
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ISSN | 2041-1723 |
出版年 | 2019 |
卷号 | 10 |
文章类型 | Article |
语种 | 英语 |
国家 | Switzerland |
英文摘要 | How microbes dynamically coordinate uptake and simultaneous utilization of nutrients in complex nutritional ecosystems is still an open question. Here, we develop a constraint-based modeling approach that exploits non-targeted exo-metabolomics data to unravel adaptive decision-making processes in dynamic nutritional environments. We thereby investigate metabolic adaptation of Escherichia coli to continuously changing conditions during batch growth in complex medium. Unexpectedly, model-based analysis of time resolved exo-metabolome data revealed that fastest growth coincides with preferred catabolism of amino acids, which, in turn, reduces glucose uptake and increases acetate overflow. We show that high intracellular levels of the amino acid degradation metabolites pyruvate and oxaloacetate can directly inhibit the phosphotransferase system (PTS), and reveal their functional role in mediating regulatory decisions for uptake and catabolism of alternative carbon sources. Overall, the proposed methodology expands the spectrum of possible applications of flux balance analysis to decipher metabolic adaptation mechanisms in naturally occurring habitats and diverse organisms. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000477705000003 |
WOS关键词 | PROTEIN-METABOLITE INTERACTIONS ; HIGH-THROUGHPUT ; OVERFLOW METABOLISM ; REVEALS PRINCIPLES ; MALIC ENZYMES ; GROWTH ; CARBON ; STATE ; QUANTIFICATION ; ALLOCATION |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/203300 |
专题 | 资源环境科学 |
作者单位 | Swiss Fed Inst Technol, Inst Mol Syst Biol, CH-8093 Zurich, Switzerland |
推荐引用方式 GB/T 7714 | Zampieri, Mattia,Hoerl, Manuel,Hotz, Florian,et al. Regulatory mechanisms underlying coordination of amino acid and glucose catabolism in Escherichia coli[J]. NATURE COMMUNICATIONS,2019,10. |
APA | Zampieri, Mattia,Hoerl, Manuel,Hotz, Florian,Mueller, Nicola F.,&Sauer, Uwe.(2019).Regulatory mechanisms underlying coordination of amino acid and glucose catabolism in Escherichia coli.NATURE COMMUNICATIONS,10. |
MLA | Zampieri, Mattia,et al."Regulatory mechanisms underlying coordination of amino acid and glucose catabolism in Escherichia coli".NATURE COMMUNICATIONS 10(2019). |
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