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DOI | 10.1038/ncomms15246 |
Aminoacyl sulfonamide assembly in SB-203208 biosynthesis | |
Hu, Zhijuan1,2; Awakawa, Takayoshi1,3; Ma, Zhongjun2; Abe, Ikuro1,3 | |
2019-01-14 | |
发表期刊 | NATURE COMMUNICATIONS |
ISSN | 2041-1723 |
出版年 | 2019 |
卷号 | 10 |
文章类型 | Article |
语种 | 英语 |
国家 | Japan; Peoples R China |
英文摘要 | Sulfonamide is present in many important drugs, due to its unique chemical and biological properties. In contrast, naturally occurring sulfonamides are rare, and their biosynthetic knowledge are scarce. Here we identify the biosynthetic gene cluster of sulfonamide antibiotics, altemicidin, SB-203207, and SB-203208, from Streptomyces sp. NCIMB40513. The heterologous gene expression and biochemical analyses reveal unique aminoacyl transfer reactions, including the tRNA synthetase-like enzyme SbzA-catalyzed L-isoleucine transfer and the GNAT enzyme SbzC-catalyzed beta-methylphenylalanine transfer. Furthermore, we elucidate the biogenesis of 2-sulfamoylacetic acid from L-cysteine, by the collaboration of the cupin dioxygenase SbzM and the aldehyde dehydrogenase SbzJ. Remarkably, SbzM catalyzes the two-step oxidation and decarboxylation of L-cysteine, and the subsequent intramolecular amino group rearrangement leads to N-S bond formation. This detailed analysis of the aminoacyl sulfonamide antibiotics biosynthetic machineries paves the way toward investigations of sulfonamide biosynthesis and its engineering. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000455595400007 |
WOS关键词 | RNA SYNTHETASE INHIBITORS ; NATURAL-PRODUCTS ; DECARBOXYLASE ; DIOXYGENASES ; SULFINES ; PATHWAY ; ENZYMES ; BOND |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/203293 |
专题 | 资源环境科学 |
作者单位 | 1.Univ Tokyo, Grad Sch Pharmaceut Sci, Bunkyo Ku, 7-3-1 Hongo, Tokyo 1130033, Japan; 2.Zhejiang Univ, Ocean Coll, Zhoushan 316000, Peoples R China; 3.Univ Tokyo, Collaborat Res Inst Innovat Microbiol, Bunkyo Ku, Yayoi 1-1-1, Tokyo 1138657, Japan |
推荐引用方式 GB/T 7714 | Hu, Zhijuan,Awakawa, Takayoshi,Ma, Zhongjun,et al. Aminoacyl sulfonamide assembly in SB-203208 biosynthesis[J]. NATURE COMMUNICATIONS,2019,10. |
APA | Hu, Zhijuan,Awakawa, Takayoshi,Ma, Zhongjun,&Abe, Ikuro.(2019).Aminoacyl sulfonamide assembly in SB-203208 biosynthesis.NATURE COMMUNICATIONS,10. |
MLA | Hu, Zhijuan,et al."Aminoacyl sulfonamide assembly in SB-203208 biosynthesis".NATURE COMMUNICATIONS 10(2019). |
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