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DOI10.1038/ncomms14996
Non-coding cis-element of Period2 is essential for maintaining organismal circadian behaviour and body temperature rhythmicity
Doi, Masao1; Shimatani, Hiroyuki1; Atobe, Yuta1; Murai, Iori1,2; Hayashi, Hida1; Takahashi, Yukari1; Fustin, Jean-Michel1; Yamaguchi, Yoshiaki1; Kiyonari, Hiroshi3; Koike, Nobuya4; Yagita, Kazuhiro4; Lee, Choogon5; Abe, Manabu6; Sakimura, Kenji6; Okamura, Hitoshi1,2
2019-06-12
发表期刊NATURE COMMUNICATIONS
ISSN2041-1723
出版年2019
卷号10
文章类型Article
语种英语
国家Japan; USA
英文摘要

Non-coding cis-regulatory elements are essential determinants of development, but their exact impacts on behavior and physiology in adults remain elusive. Cis-element-based transcriptional regulation is believed to be crucial for generating circadian rhythms in behavior and physiology. However, genetic evidence supporting this model is based on mutations in the protein-coding sequences of clock genes. Here, we report generation of mutant mice carrying a mutation only at the E'-box cis-element in the promoter region of the core clock gene Per2. The Per2 E'-box mutation abolishes sustainable molecular clock oscillations and renders circadian locomotor activity and body temperature rhythms unstable. Without the E'-box, Per2 messenger RNA and protein expression remain at mid-to-high levels. Our work delineates the Per2 E'-box as a critical nodal element for keeping sustainable cell-autonomous circadian oscillation and reveals the extent of the impact of the non-coding cis-element in daily maintenance of animal locomotor activity and body temperature rhythmicity.


领域资源环境
收录类别SCI-E
WOS记录号WOS:000471223500004
WOS关键词TRANSCRIPTIONAL ARCHITECTURE ; GENE-EXPRESSION ; STEM-CELLS ; CLOCK ; SCN ; PIGGYBAC ; OSCILLATIONS ; MUTATION ; ENHANCER ; PROTEIN
WOS类目Multidisciplinary Sciences
WOS研究方向Science & Technology - Other Topics
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文献类型期刊论文
条目标识符http://119.78.100.173/C666/handle/2XK7JSWQ/203270
专题资源环境科学
作者单位1.Kyoto Univ, Grad Sch Pharmaceut Sci, Dept Syst Biol, Sakyo Ku, Kyoto 6068501, Japan;
2.Kyoto Univ, Grad Sch Pharmaceut Sci, Lab Mol Brain Sci, Sakyo Ku, Kyoto 6068501, Japan;
3.RIKEN, Labs Anim Resource Dev & Genet Engn, Ctr Biosyst Dynam Res, Kobe, Hyogo 6500047, Japan;
4.Kyoto Prefectural Univ Med, Dept Physiol & Syst Biosci, Kyoto 6028566, Japan;
5.Florida State Univ, Coll Med, Dept Biomed Sci, Tallahassee, FL 32306 USA;
6.Niigata Univ, Brain Res Inst, Dept Cellular Neurobiol, Niigata 9518585, Japan
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Doi, Masao,Shimatani, Hiroyuki,Atobe, Yuta,et al. Non-coding cis-element of Period2 is essential for maintaining organismal circadian behaviour and body temperature rhythmicity[J]. NATURE COMMUNICATIONS,2019,10.
APA Doi, Masao.,Shimatani, Hiroyuki.,Atobe, Yuta.,Murai, Iori.,Hayashi, Hida.,...&Okamura, Hitoshi.(2019).Non-coding cis-element of Period2 is essential for maintaining organismal circadian behaviour and body temperature rhythmicity.NATURE COMMUNICATIONS,10.
MLA Doi, Masao,et al."Non-coding cis-element of Period2 is essential for maintaining organismal circadian behaviour and body temperature rhythmicity".NATURE COMMUNICATIONS 10(2019).
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