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DOI | 10.1038/ncomms14669 |
Optogenetic control of Bacillus subtilis gene expression | |
Castillo-Hair, Sebastian M.1; Baerman, Elliot A.2; Fujita, Masaya3; Igoshin, Oleg A.1,2,4; Tabor, Jeffrey J.1,2 | |
2019-07-15 | |
发表期刊 | NATURE COMMUNICATIONS |
ISSN | 2041-1723 |
出版年 | 2019 |
卷号 | 10 |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | The Gram-positive bacterium Bacillus subtilis exhibits complex spatial and temporal gene expression signals. Although optogenetic tools are ideal for studying such processes, none has been engineered for this organism. Here, we port a cyanobacterial light sensor pathway comprising the green/red photoreversible two-component system CcaSR, two metabolic enzymes for production of the chromophore phycocyanobilin (PCB), and an output promoter to control transcription of a gene of interest into B. subtilis. Following an initial non-functional design, we optimize expression of pathway genes, enhance PCB production via a translational fusion of the biosynthetic enzymes, engineer a strong chimeric output promoter, and increase dynamic range with a miniaturized photosensor kinase. Our final design exhibits over 70-fold activation and rapid response dynamics, making it well-suited to studying a wide range of gene regulatory processes. In addition, the synthetic biology methods we develop to port this pathway should make B. subtilis easier to engineer in the future. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000475469200003 |
WOS关键词 | CYANOBACTERIOCHROME CCAS ; SPATIAL-ORGANIZATION ; LIGHT ; SPORULATION ; DESIGN ; TRANSCRIPTION ; CONSTRUCTION ; TRANSLATION ; ROLES ; PHYCOCYANOBILIN |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/203269 |
专题 | 资源环境科学 |
作者单位 | 1.Rice Univ, Dept Bioengn, 6100 Main St, Houston, TX 77005 USA; 2.Rice Univ, Dept Biosci, 6100 Main St, Houston, TX 77005 USA; 3.Univ Houston, Dept Biol & Biochem, 4800 Calhoun Rd, Houston, TX 77004 USA; 4.Rice Univ, Ctr Theoret Biophys, 6100 Main St, Houston, TX 77005 USA |
推荐引用方式 GB/T 7714 | Castillo-Hair, Sebastian M.,Baerman, Elliot A.,Fujita, Masaya,et al. Optogenetic control of Bacillus subtilis gene expression[J]. NATURE COMMUNICATIONS,2019,10. |
APA | Castillo-Hair, Sebastian M.,Baerman, Elliot A.,Fujita, Masaya,Igoshin, Oleg A.,&Tabor, Jeffrey J..(2019).Optogenetic control of Bacillus subtilis gene expression.NATURE COMMUNICATIONS,10. |
MLA | Castillo-Hair, Sebastian M.,et al."Optogenetic control of Bacillus subtilis gene expression".NATURE COMMUNICATIONS 10(2019). |
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