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DOI | 10.1038/ncomms14873 |
CUTseq is a versatile method for preparing multiplexed DNA sequencing libraries from low-input samples | |
Zhang, Xiaolu1; Garnerone, Silvano1; Simonetti, Michele1; Harbers, Luuk1; Nicos, Marcin1,2; Mirzazadeh, Reza1; Venesio, Tiziana3; Sapino, Anna3,4; Hartman, Johan5,6; Marchio, Caterina3,4; Bienko, Magda1; Crosetto, Nicola1 | |
2019-10-18 | |
发表期刊 | NATURE COMMUNICATIONS |
ISSN | 2041-1723 |
出版年 | 2019 |
卷号 | 10 |
文章类型 | Article |
语种 | 英语 |
国家 | Sweden; Poland; Italy |
英文摘要 | Current multiplexing strategies for massively parallel sequencing of genomic DNA mainly rely on library indexing in the final steps of library preparation. This procedure is costly and time-consuming, because a library must be generated separately for each sample. Furthermore, library preparation is challenging in the case of fixed samples, such as DNA extracted from formalin-fixed paraffin-embedded (FFPE) tissues. Here we describe CUTseq, a method that uses restriction enzymes and in vitro transcription to barcode and amplify genomic DNA prior to library construction. We thoroughly assess the sensitivity and reproducibility of CUTseq in both cell lines and FFPE samples, and demonstrate an application of CUTseq for multi-region DNA copy number profiling within single FFPE tumor sections, to assess intratumor genetic heterogeneity at high spatial resolution. In conclusion, CUTseq is a versatile and cost-effective method for library preparation for reduced representation genome sequencing, which can find numerous applications in research and diagnostics. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000490981900001 |
WOS关键词 | BREAST-CANCER ; SINGLE-NUCLEOTIDE ; AMPLIFICATION ; EVOLUTION ; TOOLS ; IDENTIFICATION ; LANDSCAPE |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/203258 |
专题 | 资源环境科学 |
作者单位 | 1.Karolinska Inst, Dept Med Biochem & Biophys, Sci Life Lab, SE-17165 Stockholm, Sweden; 2.Med Univ Lublin, Dept Pneumonol Oncol & Allergol, PL-20954 Lublin, Poland; 3.FPO IRCCS, Candiolo Canc Inst, Pathol Unit, I-10060 Candiolo, TO, Italy; 4.Univ Turin, Dept Med Sci, Turin, Italy; 5.Karolinska Inst, Dept Oncol & Pathol, SE-17177 Stockholm, Sweden; 6.Karolinska Univ Lab, Dept Clin Pathol, S-17176 Stockholm, Sweden |
推荐引用方式 GB/T 7714 | Zhang, Xiaolu,Garnerone, Silvano,Simonetti, Michele,et al. CUTseq is a versatile method for preparing multiplexed DNA sequencing libraries from low-input samples[J]. NATURE COMMUNICATIONS,2019,10. |
APA | Zhang, Xiaolu.,Garnerone, Silvano.,Simonetti, Michele.,Harbers, Luuk.,Nicos, Marcin.,...&Crosetto, Nicola.(2019).CUTseq is a versatile method for preparing multiplexed DNA sequencing libraries from low-input samples.NATURE COMMUNICATIONS,10. |
MLA | Zhang, Xiaolu,et al."CUTseq is a versatile method for preparing multiplexed DNA sequencing libraries from low-input samples".NATURE COMMUNICATIONS 10(2019). |
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