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DOI | 10.1038/ncomms14239 |
Restoration of visual function by transplantation of optogenetically engineered photoreceptors | |
Garita-Hernandez, Marcela1; Lampic, Marusa1; Chaffiol, Antoine1; Guibbal, Laure1; Routet, Fiona1; Santos-Ferreira, Tiago2; Gasparini, Sylvia2; Borsch, Oliver2; Gagliardi, Giuliana1; Reichman, Sacha1; Picaud, Serge1; Sahel, Jose-Alain1,3,4; Goureau, Olivier1; Ader, Marius2; Dalkara, Deniz1; Duebel, Jens1,5 | |
2019-10-04 | |
发表期刊 | NATURE COMMUNICATIONS |
ISSN | 2041-1723 |
出版年 | 2019 |
卷号 | 10 |
文章类型 | Article |
语种 | 英语 |
国家 | France; Germany; USA |
英文摘要 | A major challenge in the treatment of retinal degenerative diseases, with the transplantation of replacement photoreceptors, is the difficulty in inducing the grafted cells to grow and maintain light sensitive outer segments in the host retina, which depends on proper interaction with the underlying retinal pigment epithelium (RPE). Here, for an RPE-independent treatment approach, we introduce a hyperpolarizing microbial opsin into photoreceptor precursors from newborn mice, and transplant them into blind mice lacking the photoreceptor layer. These optogenetically-transformed photoreceptors are light responsive and their transplantation leads to the recovery of visual function, as shown by ganglion cell recordings and behavioral tests. Subsequently, we generate cone photoreceptors from human induced pluripotent stem cells, expressing the chloride pump Jaws. After transplantation into blind mice, we observe light-driven responses at the photoreceptor and ganglion cell levels. These results demonstrate that structural and functional retinal repair is possible by combining stem cell therapy and optogenetics. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000489013700017 |
WOS关键词 | RETINAL-PIGMENT EPITHELIUM ; PLURIPOTENT STEM-CELLS ; HUMAN IPS CELLS ; CONE PHOTORECEPTORS ; HOST PHOTORECEPTORS ; MICE ; DEGENERATION ; HISTOPATHOLOGY ; INTEGRATION ; PRECURSORS |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/203183 |
专题 | 资源环境科学 |
作者单位 | 1.Sorbonne Univ, Inst Vis, INSERM, CNRS, F-75012 Paris, France; 2.Tech Univ Dresden, CRTD, CMCB, Dresden, Germany; 3.INSERM DGOS CIC 1423, DHU Sight Restore, CHNO Quinze Vingts, Paris, France; 4.Univ Pittsburgh, Sch Med, Dept Ophthalmol, Pittsburgh, PA 15261 USA; 5.Univ Med Ctr Gottingen, Dept Ophthalmol, Gottingen, Germany |
推荐引用方式 GB/T 7714 | Garita-Hernandez, Marcela,Lampic, Marusa,Chaffiol, Antoine,et al. Restoration of visual function by transplantation of optogenetically engineered photoreceptors[J]. NATURE COMMUNICATIONS,2019,10. |
APA | Garita-Hernandez, Marcela.,Lampic, Marusa.,Chaffiol, Antoine.,Guibbal, Laure.,Routet, Fiona.,...&Duebel, Jens.(2019).Restoration of visual function by transplantation of optogenetically engineered photoreceptors.NATURE COMMUNICATIONS,10. |
MLA | Garita-Hernandez, Marcela,et al."Restoration of visual function by transplantation of optogenetically engineered photoreceptors".NATURE COMMUNICATIONS 10(2019). |
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