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DOI | 10.1038/s41586-018-0589-x |
Visualizing late states of human 40S ribosomal subunit maturation | |
Ameismeier, Michael1,2; Cheng, Jingdong1,2; Berninghausen, Otto1; Beckmann, Roland1 | |
2018-06-14 | |
发表期刊 | NATURE |
ISSN | 0028-0836 |
EISSN | 1476-4687 |
出版年 | 2018 |
卷号 | 558期号:7709页码:249-+ |
文章类型 | Article |
语种 | 英语 |
国家 | Germany |
英文摘要 | The formation of eukaryotic ribosomal subunits extends from the nucleolus to the cytoplasm and entails hundreds of assembly factors. Despite differences in the pathways of ribosome formation, high-resolution structural information has been available only from fungi. Here we present cryo-electron microscopy structures of late-stage human 40S assembly intermediates, representing one state reconstituted in vitro and five native states that range from nuclear to late cytoplasmic. The earliest particles reveal the position of the biogenesis factor RRP12 and distinct immature rRNA conformations that accompany the formation of the 40S subunit head. Molecular models of the late-acting assembly factors TSR1, RIOK1, RIOK2, ENP1, LTV1, PNO1 and NOB1 provide mechanistic details that underlie their contribution to a sequential 40S subunit assembly. The NOB1 architecture displays an inactive nuclease conformation that requires rearrangement of the PNO1-bound 3' rRNA, thereby coordinating the final rRNA folding steps with site 3 cleavage. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000435071400045 |
WOS关键词 | CYTOPLASMIC MATURATION ; NUCLEAR EXPORT ; FINAL STEPS ; BIOGENESIS ; PROTEIN ; RIO1 ; TOOLS |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/202974 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Univ Munich, Gene Ctr Munich, Munich, Germany; 2.Univ Munich, CiPS M, Dept Biochem, Munich, Germany |
推荐引用方式 GB/T 7714 | Ameismeier, Michael,Cheng, Jingdong,Berninghausen, Otto,et al. Visualizing late states of human 40S ribosomal subunit maturation[J]. NATURE,2018,558(7709):249-+. |
APA | Ameismeier, Michael,Cheng, Jingdong,Berninghausen, Otto,&Beckmann, Roland.(2018).Visualizing late states of human 40S ribosomal subunit maturation.NATURE,558(7709),249-+. |
MLA | Ameismeier, Michael,et al."Visualizing late states of human 40S ribosomal subunit maturation".NATURE 558.7709(2018):249-+. |
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