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DOI | 10.1126/science.aay0967 |
Active site rearrangement and structural divergence in prokaryotic respiratory oxidases | |
Safarian, S.1; Hahn, A.2; Mills, D. J.2; Radloff, M.1; Eisinger, M. L.1; Nikolaev, A.3; Meier-Credo, J.1; Melin, F.3; Miyoshi, H.4; Gennis, R. B.5; Sakamoto, J.6; Langer, J. D.1; Hellwig, P.3,7; Kuehlbrandt, W.2; Michel, H.1 | |
2019-10-04 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2019 |
卷号 | 366期号:6461页码:100-+ |
文章类型 | Article |
语种 | 英语 |
国家 | Germany; France; Japan; USA |
英文摘要 | Cytochrome bd-type quinol oxidases catalyze the reduction of molecular oxygen to water in the respiratory chain of many human-pathogenic bacteria. They are structurally unrelated to mitochondrial cytochrome c oxidases and are therefore a prime target for the development of antimicrobial drugs. We determined the structure of the Escherichia coli cytochrome bd-I oxidase by single-particle cryo-electron microscopy to a resolution of 2.7 angstroms. Our structure contains a previously unknown accessory subunit CydH, the L-subfamily-specific Q-loop domain, a structural ubiquinone-8 cofactor, an active-site density interpreted as dioxygen, distinct water-filled proton channels, and an oxygen-conducting pathway. Comparison with another cytochrome bd oxidase reveals structural divergence in the family, including rearrangement of high-spin hemes and conformational adaption of a transmembrane helix to generate a distinct oxygen-binding site. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000489147000064 |
WOS关键词 | BD QUINOL OXIDASE ; HEME B(558) COMPONENT ; CYTOCHROME-C-OXIDASE ; ESCHERICHIA-COLI ; UBIQUINOL OXIDASE ; CATALYTIC CYCLE ; MECHANISM ; INTERMEDIATE ; VISUALIZATION ; OXIDATION |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/202451 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Max Planck Inst Biophys, Dept Mol Membrane Biol, D-60438 Frankfurt, Germany; 2.Max Planck Inst Biophys, Dept Struct Biol, D-60438 Frankfurt, Germany; 3.Univ Strasbourg, Lab Bioelectrochim & Spect, UMR 7140, Chim Matiere Complexe,CNRS, F-67000 Strasbourg, France; 4.Kyoto Univ, Div Appl Life Sci, Grad Sch Agr, Sakyo Ku, Kyoto 6068502, Japan; 5.Univ Illinois, Dept Biochem, Urbana, IL 61801 USA; 6.Kyushu Inst Technol, Dept Biosci & Bioinformat, Kawazu 680-4, Iizuka, Fukuoka 8208502, Japan; 7.Univ Strasbourg, Inst Adv Study, Strasbourg, France |
推荐引用方式 GB/T 7714 | Safarian, S.,Hahn, A.,Mills, D. J.,et al. Active site rearrangement and structural divergence in prokaryotic respiratory oxidases[J]. SCIENCE,2019,366(6461):100-+. |
APA | Safarian, S..,Hahn, A..,Mills, D. J..,Radloff, M..,Eisinger, M. L..,...&Michel, H..(2019).Active site rearrangement and structural divergence in prokaryotic respiratory oxidases.SCIENCE,366(6461),100-+. |
MLA | Safarian, S.,et al."Active site rearrangement and structural divergence in prokaryotic respiratory oxidases".SCIENCE 366.6461(2019):100-+. |
条目包含的文件 | 条目无相关文件。 |
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