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DOI | 10.1126/science.aaw9904 |
Time-resolved crystallography reveals allosteric communication aligned with molecular breathing | |
Mehrabi, Pedram1,2,3; Schulz, Eike C.1; Dsouza, Raison1,4; Mueller-Werkmeister, Henrike M.1,5; Tellkamp, Friedjof6; Miller, R. J. Dwayne1,7,8; Pai, Emil F.2,3,9,10 | |
2019-09-13 | |
发表期刊 | SCIENCE
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ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2019 |
卷号 | 365期号:6458页码:1167-+ |
文章类型 | Article |
语种 | 英语 |
国家 | Germany; Canada |
英文摘要 | A comprehensive understanding of protein function demands correlating structure and dynamic changes. Using time-resolved serial synchrotron crystallography, we visualized half-of-the-sites reactivity and correlated molecular-breathing motions in the enzyme fluoroacetate dehalogenase. Eighteen time points from 30 milliseconds to 30 seconds cover four turnover cycles of the irreversible reaction. They reveal sequential substrate binding, covalent-intermediate formation, setup of a hydrolytic water molecule. and product release. Small structural changes of the protein mold and variations in the number and placement of water molecules accompany the various chemical steps of catalysis. Triggered by enzyme-ligand interactions, these repetitive changes in the protein framework's dynamics and entropy constitute crucial components of the catalytic machinery. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000485780800047 |
WOS关键词 | GENERAL FORCE-FIELD ; CONFORMATIONAL-CHANGE ; ORGANOFLUORINE CHEMISTRY ; CRYSTALS ; PROTEIN ; CELL ; HETEROGENEITY ; SYNCHROTRON ; DYNAMICS ; ENZYMES |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/202306 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Max Planck Inst Struct & Dynam Matter, Dept Atomically Resolved Dynam, Luruper Chaussee 149, D-22761 Hamburg, Germany; 2.Univ Toronto, Dept Med Biophys, 101 Coll St, Toronto, ON M5G 1L7, Canada; 3.Ontario Canc Inst, Campbell Family Canc Res Inst, 101 Coll St, Toronto, ON M5G 1L7, Canada; 4.Univ Hamburg, Dept Phys, Jungiusstr 9, D-20355 Hamburg, Germany; 5.Univ Potsdam, Inst Chem Phys Chem, Karl Liebknecht Str 24-25, D-14476 Potsdam, Germany; 6.Max Planck Inst Struct & Dynam Matter, Sci Support Unit Machine Phys, Luruper Chaussee 149, D-22761 Hamburg, Germany; 7.Univ Toronto, Dept Chem, 80 St George St, Toronto, ON M5S 3H6, Canada; 8.Univ Toronto, Dept Phys, 80 St George St, Toronto, ON M5S 3H6, Canada; 9.Univ Toronto, Dept Biochem, 1 Kings Coll Circle, Toronto, ON M5S 1A8, Canada; 10.Univ Toronto, Dept Mol Genet, 1 Kings Coll Circle, Toronto, ON M5S 1A8, Canada |
推荐引用方式 GB/T 7714 | Mehrabi, Pedram,Schulz, Eike C.,Dsouza, Raison,et al. Time-resolved crystallography reveals allosteric communication aligned with molecular breathing[J]. SCIENCE,2019,365(6458):1167-+. |
APA | Mehrabi, Pedram.,Schulz, Eike C..,Dsouza, Raison.,Mueller-Werkmeister, Henrike M..,Tellkamp, Friedjof.,...&Pai, Emil F..(2019).Time-resolved crystallography reveals allosteric communication aligned with molecular breathing.SCIENCE,365(6458),1167-+. |
MLA | Mehrabi, Pedram,et al."Time-resolved crystallography reveals allosteric communication aligned with molecular breathing".SCIENCE 365.6458(2019):1167-+. |
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