Programmable CRISPR-responsive smart materials

doi:10.1126/science.aaw5122

GSTDTAP > 地球科学

DOI	10.1126/science.aaw5122
	Programmable CRISPR-responsive smart materials
	English, Max A.1,2; Soenksen, Luis R.2,3,4; Gayet, Raphael V.1,2,5; de Puig, Helena 2,4; Angenent-Mari, Nicolaas M.1,2,4; Mao, Angelo S.2,4,6; Nguyen, Peter Q.4,6; Collins, James J.1,2,4,7,8,9
	2019-08-23
发表期刊	SCIENCE
ISSN	0036-8075
EISSN	1095-9203
出版年	2019
卷号	365 期号:6455 页码:780-+
文章类型	Article
语种	英语
国家	USA
英文摘要	Stimuli-responsive materials activated by biological signals play an increasingly important role in biotechnology applications. We exploit the programmability of CRISPR-associated nucleases to actuate hydrogels containing DNA as a structural element or as an anchor for pendant groups. After activation by guide RNA-defined inputs, Cas12a cleaves DNA in the gels, thereby converting biological information into changes in material properties. We report four applications: (i) branched poly(ethylene glycol) hydrogels releasing DNA-anchored compounds, (ii) degradable polyacrylamide-DNA hydrogels encapsulating nanoparticles and live cells, (iii) conductive carbon-black-DNA hydrogels acting as degradable electrical fuses, and (iv) a polyacrylamide-DNA hydrogel operating as a fluidic valve with an electrical readout for remote signaling. These materials allow for a range of in vitro applications in tissue engineering, bioelectronics, and diagnostics.
领域	地球科学 ; 气候变化 ; 资源环境
收录类别	SCI-E
WOS记录号	WOS:000482464000046
WOS关键词	NUCLEIC-ACID DETECTION ; DNA HYDROGEL ; PLATFORM ; APTAMER ; CONDUCTIVITY ; RESISTANCE ; GRAPHENE ; RELEASE
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/202140
专题	地球科学资源环境科学气候变化
作者单位	1.MIT, Dept Biol Engn, Cambridge, MA 02139 USA; 2.MIT, Inst Med Engn & Sci, Cambridge, MA 02139 USA; 3.MIT, Dept Mech Engn, Cambridge, MA 02139 USA; 4.Harvard Univ, Wyss Inst Biologically Inspired Engn, Boston, MA 02115 USA; 5.MIT, Microbiol Grad Program, Cambridge, MA 02139 USA; 6.Harvard Univ, Sch Engn & Appl Sci, Cambridge, MA 02138 USA; 7.MIT, Synthet Biol Ctr, Cambridge, MA 02139 USA; 8.MIT & Harvard, Broad Inst, Cambridge, MA 02142 USA; 9.Harvard Program MIT Hlth Sci & Technol, Cambridge, MA 02139 USA
推荐引用方式 GB/T 7714	English, Max A.,Soenksen, Luis R.,Gayet, Raphael V.,et al. Programmable CRISPR-responsive smart materials[J]. SCIENCE,2019,365(6455):780-+.
APA	English, Max A..,Soenksen, Luis R..,Gayet, Raphael V..,de Puig, Helena.,Angenent-Mari, Nicolaas M..,...&Collins, James J..(2019).Programmable CRISPR-responsive smart materials.SCIENCE,365(6455),780-+.
MLA	English, Max A.,et al."Programmable CRISPR-responsive smart materials".SCIENCE 365.6455(2019):780-+.