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DOI | 10.1126/science.aax4240 |
Phospho-dependent phase separation of FMRP and CAPRIN1 recapitulates regulation of translation and deadenylation | |
Kim, Tae Hun1,2,3,4; Tsang, Brian1,2; Vernon, Robert M.1; Sonenberg, Nahum5,6; Kay, Lewis E.1,2,3,4; Forman-Kay, Julie D.1,2 | |
2019-08-23 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2019 |
卷号 | 365期号:6455页码:825-+ |
文章类型 | Article |
语种 | 英语 |
国家 | Canada |
英文摘要 | Membraneless organelles involved in RNA processing are biomolecular condensates assembled by phase separation. Despite the important role of intrinsically disordered protein regions (IDRs), the specific interactions underlying IDR phase separation and its functional consequences remain elusive. To address these questions, we used minimal condensates formed from the C-terminal disordered regions of two interacting translational regulators, FMRP and CAPRIN1. Nuclear magnetic resonance spectroscopy of FMRP-CAPRIN1 condensates revealed interactions involving arginine-rich and aromatic-rich regions. We found that different FMRP serine/threonine and CAPRIN1 tyrosine phosphorylation patterns control phase separation propensity with RNA, including subcompartmentalization, and tune deadenylation and translation rates in vitro. The resulting evidence for residue-specific interactions underlying co-phase separation. phosphorylation-modulated condensate architecture, and enzymatic activity within condensates has implications for how the integration of signaling pathways controls RNA processing and translation. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000482464000055 |
WOS关键词 | NEURONAL RNA GRANULES ; NMR-SPECTROSCOPY ; STRESS GRANULES ; PROTEIN ; CONDENSATION ; RECOGNITION ; REVEALS ; MEDIATE ; COMPLEX ; REGION |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/202133 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Hosp Sick Children, Program Mol Med, Toronto, ON M5G 0A4, Canada; 2.Univ Toronto, Dept Biochem, Toronto, ON M5S 1A8, Canada; 3.Univ Toronto, Dept Mol Genet, Toronto, ON M5S 1A8, Canada; 4.Univ Toronto, Dept Chem, Toronto, ON M5S 1A8, Canada; 5.McGill Univ, Dept Biochem, Montreal, PQ H3G 1Y6, Canada; 6.McGill Univ, Goodman Canc Res Ctr, Montreal, PQ H3A 1A3, Canada |
推荐引用方式 GB/T 7714 | Kim, Tae Hun,Tsang, Brian,Vernon, Robert M.,et al. Phospho-dependent phase separation of FMRP and CAPRIN1 recapitulates regulation of translation and deadenylation[J]. SCIENCE,2019,365(6455):825-+. |
APA | Kim, Tae Hun,Tsang, Brian,Vernon, Robert M.,Sonenberg, Nahum,Kay, Lewis E.,&Forman-Kay, Julie D..(2019).Phospho-dependent phase separation of FMRP and CAPRIN1 recapitulates regulation of translation and deadenylation.SCIENCE,365(6455),825-+. |
MLA | Kim, Tae Hun,et al."Phospho-dependent phase separation of FMRP and CAPRIN1 recapitulates regulation of translation and deadenylation".SCIENCE 365.6455(2019):825-+. |
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