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DOI | 10.1126/science.aav6416 |
Bright and photostable chemigenetic indicators for extended in vivo voltage imaging | |
Abdelfattah, Ahmed S.1; Kawashima, Takashi1,11; Singh, Amrita1,2; Novak, Ondrej1,3; Liu, Hui1; Shuai, Yichun1; Huang, Yi-Chieh4; Campagnola, Luke5; Seeman, Stephanie C.5; Yu, Jianing1; Zheng, Jihong1; Grimm, Jonathan B.1; Patel, Ronak1; Friedrich, Johannes6,7,8,9,10; Mensh, Brett D.1; Paninski, Liam6,7,8,9; Macklin, John J.1; Murphy, Gabe J.5; Podgorski, Kaspar1; Lin, Bei-Jung4; Chen, Tsai-Wen4; Turner, Glenn C.1; Liu, Zhe1; Koyama, Minoru1; Svoboda, Karel1; Ahrens, Misha B.1; Lavis, Luke D.1; Schreiter, Eric R.1 | |
2019-08-16 | |
发表期刊 | SCIENCE
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ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2019 |
卷号 | 365期号:6454页码:699-+ |
文章类型 | Article |
语种 | 英语 |
国家 | USA; Czech Republic; Taiwan; Israel |
英文摘要 | Genetically encoded voltage indicators (GEVIs) enable monitoring of neuronal activity at high spatial and temporal resolution. However, the utility of existing GEVIs has been limited by the brightness and photostability of fluorescent proteins and rhodopsins. We engineered a GEVI, called Voltron, that uses bright and photostable synthetic dyes instead of protein-based fluorophores, thereby extending the number of neurons imaged simultaneously in vivo by a factor of 10 and enabling imaging for significantly longer durations relative to existing GEVIs. We used Voltron for in vivo voltage imaging in mice, zebrafish, and fruit flies. In the mouse cortex, Voltron allowed single-trial recording of spikes and subthreshold voltage signals from dozens of neurons simultaneously over a 15-minute period of continuous imaging. In larval zebrafish, Voltron enabled the precise correlation of spike timing with behavior. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000481688700049 |
WOS关键词 | RED FLUORESCENT PROTEIN ; GENERAL-METHOD ; PHOTOBLEACHING KINETICS ; ACTION-POTENTIALS ; LIVE-CELL ; ZEBRAFISH ; NEURONS ; FLUOROPHORES ; SPIKES ; DYNAMICS |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/202095 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Howard Hughes Med Inst, Janelia Res Campus, Ashburn, VA 20147 USA; 2.Johns Hopkins Univ, Solomon H Snyder Dept Neurosci, Baltimore, MD 21205 USA; 3.Acad Sci Czech Republ, Inst Expt Med, Dept Auditory Neurosci, Prague, Czech Republic; 4.Natl Yang Ming Univ, Inst Neurosci, Taipei 112, Taiwan; 5.Allen Inst Brain Sci, Seattle, WA 98109 USA; 6.Columbia Univ, Dept Stat, New York, NY 10027 USA; 7.Columbia Univ, Ctr Theoret Neurosci, New York, NY 10027 USA; 8.Columbia Univ, Dept Neurosci, New York, NY 10027 USA; 9.Columbia Univ, Grossman Ctr Stat Mind, New York, NY 10027 USA; 10.Flatiron Inst, Ctr Computat Biol, New York, NY 10010 USA; 11.Weizmann Inst Sci, Dept Neurobiol, Rehovot, Israel |
推荐引用方式 GB/T 7714 | Abdelfattah, Ahmed S.,Kawashima, Takashi,Singh, Amrita,et al. Bright and photostable chemigenetic indicators for extended in vivo voltage imaging[J]. SCIENCE,2019,365(6454):699-+. |
APA | Abdelfattah, Ahmed S..,Kawashima, Takashi.,Singh, Amrita.,Novak, Ondrej.,Liu, Hui.,...&Schreiter, Eric R..(2019).Bright and photostable chemigenetic indicators for extended in vivo voltage imaging.SCIENCE,365(6454),699-+. |
MLA | Abdelfattah, Ahmed S.,et al."Bright and photostable chemigenetic indicators for extended in vivo voltage imaging".SCIENCE 365.6454(2019):699-+. |
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