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DOI | 10.1126/science.aav5477 |
Synthetic sequence entanglement augments stability and containment of genetic information in cells | |
Blazejewski, Tomasz1,2; Ho, Hsing-I1; Wang, Harris H.1,3 | |
2019-08-09 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2019 |
卷号 | 365期号:6453页码:595-+ |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | In synthetic biology, methods for stabilizing genetically engineered functions and confining recombinant DNA to intended hosts are necessary to cope with natural mutation accumulation and pervasive lateral gene flow. We present a generalizable strategy to preserve and constrain genetic information through the computational design of overlapping genes. Overlapping a sequence with an essential gene altered its fitness landscape and produced a constrained evolutionary path, even for synonymous mutations. Embedding a toxin gene in a gene of interest restricted its horizontal propagation. We further demonstrated a multiplex and scalable approach to build and test > 7500 overlapping sequence designs, yielding functional yet highly divergent variants from natural homologs. This work enables deeper exploration of natural and engineered overlapping genes and facilitates enhanced genetic stability and biocontainment in emerging applications. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000483195200041 |
WOS关键词 | OVERLAPPING GENES ; PROTEIN FOLD ; RNA ; PREDICTION ; EVOLUTION ; PRECISE |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/202051 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Columbia Univ, Dept Syst Biol, New York, NY 10027 USA; 2.Columbia Univ, Integrated Program Cellular Mol & Biomed Studies, New York, NY USA; 3.Columbia Univ, Dept Pathol & Cell Biol, New York, NY 10027 USA |
推荐引用方式 GB/T 7714 | Blazejewski, Tomasz,Ho, Hsing-I,Wang, Harris H.. Synthetic sequence entanglement augments stability and containment of genetic information in cells[J]. SCIENCE,2019,365(6453):595-+. |
APA | Blazejewski, Tomasz,Ho, Hsing-I,&Wang, Harris H..(2019).Synthetic sequence entanglement augments stability and containment of genetic information in cells.SCIENCE,365(6453),595-+. |
MLA | Blazejewski, Tomasz,et al."Synthetic sequence entanglement augments stability and containment of genetic information in cells".SCIENCE 365.6453(2019):595-+. |
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