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DOI | 10.1126/science.aax0486 |
Structure of the Cdc48 segregase in the act of unfolding an authentic substrate | |
Cooney, Ian1; Han, Han1; Stewart, Michael G.1; Carson, Richard H.2; Hansen, Daniel T.1; Iwasa, Janet H.1; Price, John C.2; Hill, Christopher P.1; Shen, Peter S.1 | |
2019-08-02 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2019 |
卷号 | 365期号:6452页码:502-+ |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | The cellular machine Cdc48 functions in multiple biological pathways by segregating its protein substrates from a variety of stable environments such as organelles or multi-subunit complexes. Despite extensive studies, the mechanismof Cdc48 has remained obscure, and its reported structures are inconsistent with models of substrate translocation proposed for other AAA+ ATPases (adenosine triphosphatases). Here, we report a 3.7-angstrom-resolution structure of Cdc48 in complex with an adaptor protein and a native substrate. Cdc48 engages substrate by adopting a helical configuration of substrate-binding residues that extends through the central pore of both of the ATPase rings. These findings indicate a unified hand-over-hand mechanism of protein translocation by Cdc48 and other AAA+ ATPases. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000485768400049 |
WOS关键词 | CRYO-EM STRUCTURE ; AAA ATPASE ; P97 ; SYSTEM ; MECHANISM ; FEATURES ; BINDING ; P97/VCP |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/201993 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Univ Utah, Dept Biochem, 15 N Med Dr East, Salt Lake City, UT 84112 USA; 2.Brigham Young Univ, Dept Chem & Biochem, C100 BNSN, Provo, UT 84602 USA |
推荐引用方式 GB/T 7714 | Cooney, Ian,Han, Han,Stewart, Michael G.,et al. Structure of the Cdc48 segregase in the act of unfolding an authentic substrate[J]. SCIENCE,2019,365(6452):502-+. |
APA | Cooney, Ian.,Han, Han.,Stewart, Michael G..,Carson, Richard H..,Hansen, Daniel T..,...&Shen, Peter S..(2019).Structure of the Cdc48 segregase in the act of unfolding an authentic substrate.SCIENCE,365(6452),502-+. |
MLA | Cooney, Ian,et al."Structure of the Cdc48 segregase in the act of unfolding an authentic substrate".SCIENCE 365.6452(2019):502-+. |
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