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DOI | 10.1126/science.aaw9128 |
Rotary substates of mitochondrial ATP synthase reveal the basis of flexible F-1-F-o coupling | |
Murphy, Bonnie J.1; Klusch, Niklas1; Langer, Julian2; Mills, Deryck J.1; Yildiz, Oezkan1; Kuehlbrandt, Werner1 | |
2019-06-21 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2019 |
卷号 | 364期号:6446页码:1155-+ |
文章类型 | Article |
语种 | 英语 |
国家 | Germany |
英文摘要 | F1Fo-adenosine triphosphate (ATP) synthases make the energy of the proton-motive force available for energy-consuming processes in the cell. We determined the single-particle cryo-electron microscopy structure of active dimeric ATP synthase from mitochondria of Polytomella sp. at a resolution of 2.7 to 2.8 angstroms. Separation of 13 well-defined rotary substates by three-dimensional classification provides a detailed picture of the molecular motions that accompany c-ring rotation and result in ATP synthesis. Crucially, the F-1 head rotates along with the central stalk and c-ring rotor for the first similar to 30 degrees of each 120 degrees primary rotary step to facilitate flexible coupling of the stoichiometrically mismatched F-1 and F-o subcomplexes. Flexibility is mediated primarily by the interdomain hinge of the conserved OSCP subunit. A conserved metal ion in the proton access channel may synchronize c-ring protonation with rotation. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000472175100035 |
WOS关键词 | CRYO-EM ; RAT-BRAIN ; SUBUNIT ; PROTEIN ; ROTATION ; COMPLEX ; IDENTIFICATION ; VISUALIZATION ; VALIDATION ; F-1-ATPASE |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/201697 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Max Planck Inst Biophys, Dept Struct Biol, D-60438 Frankfurt, Germany; 2.Max Planck Inst Biophys, Dept Mol Membrane Biol, D-60438 Frankfurt, Germany |
推荐引用方式 GB/T 7714 | Murphy, Bonnie J.,Klusch, Niklas,Langer, Julian,et al. Rotary substates of mitochondrial ATP synthase reveal the basis of flexible F-1-F-o coupling[J]. SCIENCE,2019,364(6446):1155-+. |
APA | Murphy, Bonnie J.,Klusch, Niklas,Langer, Julian,Mills, Deryck J.,Yildiz, Oezkan,&Kuehlbrandt, Werner.(2019).Rotary substates of mitochondrial ATP synthase reveal the basis of flexible F-1-F-o coupling.SCIENCE,364(6446),1155-+. |
MLA | Murphy, Bonnie J.,et al."Rotary substates of mitochondrial ATP synthase reveal the basis of flexible F-1-F-o coupling".SCIENCE 364.6446(2019):1155-+. |
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