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DOI | 10.1126/science.aaw7446 |
Bacterial pseudokinase catalyzes protein polyglutamylation to inhibit the SidE-family ubiquitin ligases | |
Black, Miles H.1; Osinski, Adam1; Gradowski, Marcin2; Servage, Kelly A.1,3; Pawlowski, Krzysztof2,4; Tomchick, Diana R.5,6; Tagliabracci, Vincent S.1,7,8 | |
2019-05-24 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2019 |
卷号 | 364期号:6442页码:787-+ |
文章类型 | Article |
语种 | 英语 |
国家 | USA; Poland; Sweden |
英文摘要 | Enzymes with a protein kinase fold transfer phosphate from adenosine 5'-triphosphate (ATP) to substrates in a process known as phosphorylation. Here, we show that the Legionella meta-effector SidJ adopts a protein kinase fold, yet unexpectedly catalyzes protein polyglutamylation. SidJ is activated by host-cell calmodulin to polyglutamylate the SidE family of ubiquitin (Ub) ligases. Crystal structures of the SidJ-calmodulin complex reveal a protein kinase fold that catalyzes ATP-dependent isopeptide bond formation between the amino group of free glutamate and the g-carboxyl group of an active-site glutamate in SidE. We show that SidJ polyglutamylation of SidE, and the consequent inactivation of Ub ligase activity, is required for successful Legionella replication in a viable eukaryotic host cell. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000469296000046 |
WOS关键词 | LEGIONELLA-PNEUMOPHILA ; ENDOPLASMIC-RETICULUM ; INTRACELLULAR GROWTH ; MODEL ; PREDICTION ; SOFTWARE ; FEATURES ; REGIONS |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/201476 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Univ Texas Southwestern Med Ctr Dallas, Dept Mol Biol, Dallas, TX 75390 USA; 2.Warsaw Univ Life Sci, Warsaw, Poland; 3.Howard Hughes Med Inst, Dallas, TX 75390 USA; 4.Lund Univ, Lund, Sweden; 5.Univ Texas Southwestern Med Ctr Dallas, Dept Biophys, Dallas, TX 75390 USA; 6.Univ Texas Southwestern Med Ctr Dallas, Dept Biochem, Dallas, TX 75390 USA; 7.Univ Texas Southwestern Med Ctr Dallas, Harold C Simmons Comprehens Canc Ctr, Dallas, TX 75390 USA; 8.Univ Texas Southwestern Med Ctr Dallas, Hamon Ctr Regenerat Sci & Med, Dallas, TX 75390 USA |
推荐引用方式 GB/T 7714 | Black, Miles H.,Osinski, Adam,Gradowski, Marcin,et al. Bacterial pseudokinase catalyzes protein polyglutamylation to inhibit the SidE-family ubiquitin ligases[J]. SCIENCE,2019,364(6442):787-+. |
APA | Black, Miles H..,Osinski, Adam.,Gradowski, Marcin.,Servage, Kelly A..,Pawlowski, Krzysztof.,...&Tagliabracci, Vincent S..(2019).Bacterial pseudokinase catalyzes protein polyglutamylation to inhibit the SidE-family ubiquitin ligases.SCIENCE,364(6442),787-+. |
MLA | Black, Miles H.,et al."Bacterial pseudokinase catalyzes protein polyglutamylation to inhibit the SidE-family ubiquitin ligases".SCIENCE 364.6442(2019):787-+. |
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