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DOI | 10.1126/science.aau8287 |
Complex signal processing in synthetic gene circuits using cooperative regulatory assemblies | |
Bashor, Caleb J.1; Patel, Nikit2,3; Choubey, Sandeep4; Beyzavi, Ali5; Kondev, Jane4; Collins, James J.6,7,8,9; Khalil, Ahmad S.2,3,9 | |
2019-05-10 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2019 |
卷号 | 364期号:6440页码:593-+ |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | Eukaryotic genes are regulated by multivalent transcription factor complexes. Through cooperative self-assembly, these complexes perform nonlinear regulatory operations involved in cellular decision-making and signal processing. In this study, we apply this design principle to synthetic networks, testing whether engineered cooperative assemblies can program nonlinear gene circuit behavior in yeast. Using a model-guided approach, we show that specifying the strength and number of assembly subunits enables predictive tuning between linear and nonlinear regulatory responses for single- and multi-input circuits. We demonstrate that assemblies can be adjusted to control circuit dynamics. We harness this capability to engineer circuits that perform dynamic filtering, enabling frequency-dependent decoding in cell populations. Programmable cooperative assembly provides a versatile way to tune the nonlinearity of network connections, markedly expanding the engineerable behaviors available to synthetic circuits. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000467631800044 |
WOS关键词 | TRANSCRIPTIONAL REGULATION ; BINDING ; COMBINATORIAL ; BIOLOGY ; INFORMATION ; SPECIFICITY ; EXPRESSION ; PRINCIPLES ; AFFINITY ; NUMBERS |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/201399 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Rice Univ, Dept Bioengn, Houston, TX 77030 USA; 2.Boston Univ, Dept Biomed Engn, Boston, MA 02215 USA; 3.Boston Univ, Biol Design Ctr, Boston, MA 02215 USA; 4.Brandeis Univ, Dept Phys, Waltham, MA 02453 USA; 5.Boston Univ, Dept Mech Engn, Boston, MA 02215 USA; 6.MIT, Inst Med Engn & Sci, Dept Biol Engn, 77 Massachusetts Ave, Cambridge, MA 02139 USA; 7.MIT, Synthet Biol Ctr, 77 Massachusetts Ave, Cambridge, MA 02139 USA; 8.Broad Inst MIT & Harvard, Cambridge, MA 02142 USA; 9.Harvard Univ, Wyss Inst Biol Inspired Engn, Boston, MA 02115 USA |
推荐引用方式 GB/T 7714 | Bashor, Caleb J.,Patel, Nikit,Choubey, Sandeep,et al. Complex signal processing in synthetic gene circuits using cooperative regulatory assemblies[J]. SCIENCE,2019,364(6440):593-+. |
APA | Bashor, Caleb J..,Patel, Nikit.,Choubey, Sandeep.,Beyzavi, Ali.,Kondev, Jane.,...&Khalil, Ahmad S..(2019).Complex signal processing in synthetic gene circuits using cooperative regulatory assemblies.SCIENCE,364(6440),593-+. |
MLA | Bashor, Caleb J.,et al."Complex signal processing in synthetic gene circuits using cooperative regulatory assemblies".SCIENCE 364.6440(2019):593-+. |
条目包含的文件 | 条目无相关文件。 |
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