Long-term functional maintenance of primary human hepatocytes in vitro

doi:10.1126/science.aau7307

GSTDTAP > 地球科学

DOI	10.1126/science.aau7307
	Long-term functional maintenance of primary human hepatocytes in vitro
	Xiang, Chengang 1,2; Du, Yuanyuan 1,2; Meng, Gaofan 1,2; Yi, Liew Soon 1,2; Sun, Shicheng 1,2; Song, Nan 1,2; Zhang, Xiaonan 3; Xiao, Yiwei 4; Wang, Jie 4; Yi, Zhigang 5; Liu, Yifang 6; Xie, Bingqing 7; Wu, Min 3; Shu, Jun 5; Sun, Da 7; Jia, Jun 1,2; Liang, Zhen 7; Sun, Dong 1,2; Huang, Yanxiang 8; Shi, Yan 1,2; Xu, Jun 1,2; Lu, Fengmin 4; Li, Cheng 6; Xiang, Kuanhui 4; Yuan, Zhenghong 5; Lu, Shichun 9; Deng, Hongkui 1,2,7
	2019-04-26
发表期刊	SCIENCE
ISSN	0036-8075
EISSN	1095-9203
出版年	2019
卷号	364 期号:6438 页码:399-+
文章类型	Article
语种	英语
国家	Peoples R China
英文摘要	The maintenance of terminally differentiated cells, especially hepatocytes, in vitro has proven challenging. Here we demonstrated the long-term in vitro maintenance of primary human hepatocytes (PHHs) by modulating cell signaling pathways with a combination of five chemicals (5C). 5C-cultured PHHs showed global gene expression profiles and hepatocyte-specific functions resembling those of freshly isolated counterparts. Furthermore, these cells efficiently recapitulated the entire course of hepatitis B virus (HBV) infection over 4 weeks with the production of infectious viral particles and formation of HBV covalently closed circular DNA. Our study demonstrates that, with a chemical approach, functional maintenance of PHHs supports long-term HBV infection in vitro, providing an efficient platform for investigating HBV cell biology and antiviral drug screening.
领域	地球科学 ; 气候变化 ; 资源环境
收录类别	SCI-E
WOS记录号	WOS:000465643400050
WOS关键词	HEPATITIS-C VIRUS ; STRATEGIES ; MECHANISMS
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/201302
专题	地球科学资源环境科学气候变化
作者单位	1.Peking Univ, Hlth Sci Ctr, State Key Lab Nat & Biomimet Drugs, Sch Basic Med Sci, Beijing 100191, Peoples R China; 2.Peking Univ, Peking Tsinghua Ctr Life Sci, Coll Life Sci, MOE Key Lab Cell Proliferat & Differentiat, Beijing 100191, Peoples R China; 3.Shanghai Publ Hlth Clin Ctr, Shanghai 201508, Peoples R China; 4.Peking Univ, Sch Basic Med Sci, Hlth Sci Ctr, Beijing 100191, Peoples R China; 5.Fudan Univ, Shanghai Med Coll, Key Lab Med Mol Virol, Shanghai 200032, Peoples R China; 6.Peking Univ, Ctr Bioinformat, Beijing 100871, Peoples R China; 7.Peking Univ, Shenzhen Grad Sch, State Key Lab Chem Oncogen, Sch Chem Biol & Biotechnol, Shenzhen 518055, Guangdong, Peoples R China; 8.Youan Hosp, Beijing 100069, Peoples R China; 9.Chinese Peoples Liberat Army Gen Hosp, Dept Hepatobiliary Surg, Beijing 100853, Peoples R China
推荐引用方式 GB/T 7714	Xiang, Chengang,Du, Yuanyuan,Meng, Gaofan,et al. Long-term functional maintenance of primary human hepatocytes in vitro[J]. SCIENCE,2019,364(6438):399-+.
APA	Xiang, Chengang.,Du, Yuanyuan.,Meng, Gaofan.,Yi, Liew Soon.,Sun, Shicheng.,...&Deng, Hongkui.(2019).Long-term functional maintenance of primary human hepatocytes in vitro.SCIENCE,364(6438),399-+.
MLA	Xiang, Chengang,et al."Long-term functional maintenance of primary human hepatocytes in vitro".SCIENCE 364.6438(2019):399-+.