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DOI | 10.1126/science.aau7307 |
Long-term functional maintenance of primary human hepatocytes in vitro | |
Xiang, Chengang1,2; Du, Yuanyuan1,2; Meng, Gaofan1,2; Yi, Liew Soon1,2; Sun, Shicheng1,2; Song, Nan1,2; Zhang, Xiaonan3; Xiao, Yiwei4; Wang, Jie4; Yi, Zhigang5; Liu, Yifang6; Xie, Bingqing7; Wu, Min3; Shu, Jun5; Sun, Da7; Jia, Jun1,2; Liang, Zhen7; Sun, Dong1,2; Huang, Yanxiang8; Shi, Yan1,2; Xu, Jun1,2; Lu, Fengmin4; Li, Cheng6; Xiang, Kuanhui4; Yuan, Zhenghong5; Lu, Shichun9; Deng, Hongkui1,2,7 | |
2019-04-26 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2019 |
卷号 | 364期号:6438页码:399-+ |
文章类型 | Article |
语种 | 英语 |
国家 | Peoples R China |
英文摘要 | The maintenance of terminally differentiated cells, especially hepatocytes, in vitro has proven challenging. Here we demonstrated the long-term in vitro maintenance of primary human hepatocytes (PHHs) by modulating cell signaling pathways with a combination of five chemicals (5C). 5C-cultured PHHs showed global gene expression profiles and hepatocyte-specific functions resembling those of freshly isolated counterparts. Furthermore, these cells efficiently recapitulated the entire course of hepatitis B virus (HBV) infection over 4 weeks with the production of infectious viral particles and formation of HBV covalently closed circular DNA. Our study demonstrates that, with a chemical approach, functional maintenance of PHHs supports long-term HBV infection in vitro, providing an efficient platform for investigating HBV cell biology and antiviral drug screening. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000465643400050 |
WOS关键词 | HEPATITIS-C VIRUS ; STRATEGIES ; MECHANISMS |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/201302 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Peking Univ, Hlth Sci Ctr, State Key Lab Nat & Biomimet Drugs, Sch Basic Med Sci, Beijing 100191, Peoples R China; 2.Peking Univ, Peking Tsinghua Ctr Life Sci, Coll Life Sci, MOE Key Lab Cell Proliferat & Differentiat, Beijing 100191, Peoples R China; 3.Shanghai Publ Hlth Clin Ctr, Shanghai 201508, Peoples R China; 4.Peking Univ, Sch Basic Med Sci, Hlth Sci Ctr, Beijing 100191, Peoples R China; 5.Fudan Univ, Shanghai Med Coll, Key Lab Med Mol Virol, Shanghai 200032, Peoples R China; 6.Peking Univ, Ctr Bioinformat, Beijing 100871, Peoples R China; 7.Peking Univ, Shenzhen Grad Sch, State Key Lab Chem Oncogen, Sch Chem Biol & Biotechnol, Shenzhen 518055, Guangdong, Peoples R China; 8.Youan Hosp, Beijing 100069, Peoples R China; 9.Chinese Peoples Liberat Army Gen Hosp, Dept Hepatobiliary Surg, Beijing 100853, Peoples R China |
推荐引用方式 GB/T 7714 | Xiang, Chengang,Du, Yuanyuan,Meng, Gaofan,et al. Long-term functional maintenance of primary human hepatocytes in vitro[J]. SCIENCE,2019,364(6438):399-+. |
APA | Xiang, Chengang.,Du, Yuanyuan.,Meng, Gaofan.,Yi, Liew Soon.,Sun, Shicheng.,...&Deng, Hongkui.(2019).Long-term functional maintenance of primary human hepatocytes in vitro.SCIENCE,364(6438),399-+. |
MLA | Xiang, Chengang,et al."Long-term functional maintenance of primary human hepatocytes in vitro".SCIENCE 364.6438(2019):399-+. |
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