Ligand-triggered allosteric ADP release primes a plant NLR complex

doi:10.1126/science.aav5868

GSTDTAP > 地球科学

DOI	10.1126/science.aav5868
	Ligand-triggered allosteric ADP release primes a plant NLR complex
	Wang, Jizong 1,2; Wang, Jia 2; Hu, Meijuan 1; Wu, Shan 2; Qi, Jinfeng 1; Wang, Guoxun 1; Han, Zhifu 2; Qi, Yijun 2; Gao, Ning 2,5; Wang, Hong-Wei 2; Zhou, Jian-Min 1; Chai, Jijie 2,3,4
	2019-04-05
发表期刊	SCIENCE
ISSN	0036-8075
EISSN	1095-9203
出版年	2019
卷号	364 期号:6435 页码:43-+
文章类型	Article
语种	英语
国家	Peoples R China; Germany
英文摘要	Pathogen recognition by nucleotide-binding (NB), leucine-rich repeat (LRR) receptors (NLRs) plays roles in plant immunity. The Xanthomonas campestris pv. campestris effector AvrAC uridylylates the Arabidopsis PBL2 kinase, and the latter (PBL2(UMP)) acts as a ligand to activate the NLR ZAR1 precomplexed with the RKS1 pseudokinase. Here we report the cryo-electron microscopy structures of ZAR1-RKS1 and ZAR1-RKS1-PBL2(UMP) in an inactive and intermediate state, respectively. The ZAR1(LRR) domain, compared with animal NLRLRR domains, is differently positioned to sequester ZAR1 in an inactive state. Recognition of PBL2(UMP) is exclusively through RKS1, which interacts with ZAR1(LRR). PBL2(UMP) binding stabilizes the RKS1 activation segment, which sterically blocks ZAR1 adenosine diphosphate (ADP) binding. This engenders a more flexible NB domain without conformational changes in the other ZAR1 domains. Our study provides a structural template for understanding plant NLRs.
领域	地球科学 ; 气候变化 ; 资源环境
收录类别	SCI-E
WOS记录号	WOS:000463585700028
WOS关键词	CRYO-EM STRUCTURE ; RUST RESISTANCE PROTEIN ; CRYSTAL-STRUCTURE ; STRUCTURAL BASIS ; RHO GTPASES ; GENE ; REVEALS ; RECOGNITION ; APOPTOSOME ; EFFECTOR
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/201131
专题	地球科学资源环境科学气候变化
作者单位	1.Chinese Acad Sci, Acad Seed Design, Inst Genet & Dev Biol, State Key Lab Plant Genom, Beijing 100101, Peoples R China; 2.Tsinghua Univ, Beijing Adv Innovat Ctr Struct Biol, Tsinghua Peking Joint Ctr Life Sci, Ctr Plant Biol,Sch Life Sci, Beijing 100084, Peoples R China; 3.Max Planck Inst Plant Breeding Res, D-50829 Cologne, Germany; 4.Univ Cologne, Inst Biochem, Zuelpicher Str 47, D-50674 Cologne, Germany; 5.Peking Univ, Sch Life Sci, Beijing 100871, Peoples R China
推荐引用方式 GB/T 7714	Wang, Jizong,Wang, Jia,Hu, Meijuan,et al. Ligand-triggered allosteric ADP release primes a plant NLR complex[J]. SCIENCE,2019,364(6435):43-+.
APA	Wang, Jizong.,Wang, Jia.,Hu, Meijuan.,Wu, Shan.,Qi, Jinfeng.,...&Chai, Jijie.(2019).Ligand-triggered allosteric ADP release primes a plant NLR complex.SCIENCE,364(6435),43-+.
MLA	Wang, Jizong,et al."Ligand-triggered allosteric ADP release primes a plant NLR complex".SCIENCE 364.6435(2019):43-+.