Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1126/science.aav7003 |
Structures and operating principles of the replisome | |
Gao, Yang1; Cui, Yanxiang2; Fox, Tara3,4; Lin, Shiqiang1,5; Wang, Huaibin1; de Val, Natalia3,4; Zhou, Z. Hong2; Yang, Wei1 | |
2019-02-22 | |
发表期刊 | SCIENCE
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ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2019 |
卷号 | 363期号:6429页码:835-+ |
文章类型 | Article |
语种 | 英语 |
国家 | USA; Peoples R China |
英文摘要 | Visualization in atomic detail of the replisome that performs concerted leading- and lagging-DNA strand synthesis at a replication fork has not been reported. Using bacteriophage T7 as a model system, we determined cryo-electron microscopy structures up to 3.2-angstroms resolution of helicase translocating along DNA and of helicase-polymerase-primase complexes engaging in synthesis of both DNA strands. Each domain of the spiral-shaped hexameric helicase translocates sequentially hand-over-hand along a single-stranded DNA coil, akin to the way AAA+ ATPases (adenosine triphosphatases) unfold peptides. Two lagging-strand polymerases are attached to the primase, ready for Okazaki fragment synthesis in tandem. A beta hairpin from the leading-strand polymerase separates two parental DNA strands into a T-shaped fork, thus enabling the closely coupled helicase to advance perpendicular to the downstream DNA duplex. These structures reveal the molecular organization and operating principles of a replisome. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000459387100034 |
WOS关键词 | BACTERIOPHAGE T7 PRIMASE ; CRYO-EM STRUCTURE ; CRYSTAL-STRUCTURE ; REPLICATION-FORK ; DNA-BINDING ; HEXAMERIC HELICASE ; PRIMER SYNTHESIS ; SINGLE-MOLECULE ; POLYMERASE ; TRANSLOCATION |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/200809 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.NIDDK, Lab Mol Biol, NIH, Bethesda, MD 20892 USA; 2.Univ Calif Los Angeles, Calif NanoSyst Inst, Los Angeles, CA 90095 USA; 3.NCI, Ctr Mol Microscopy, Ctr Canc Res, NIH, Bethesda, MD 20892 USA; 4.Leidos Biomed Res Inc, Frederick Natl Lab Canc Res, Canc Res Technol Program, Frederick, MD 21701 USA; 5.Fujian Agr & Forestry Univ, Coll Life Sci, Fuzhou, Fujian, Peoples R China |
推荐引用方式 GB/T 7714 | Gao, Yang,Cui, Yanxiang,Fox, Tara,et al. Structures and operating principles of the replisome[J]. SCIENCE,2019,363(6429):835-+. |
APA | Gao, Yang.,Cui, Yanxiang.,Fox, Tara.,Lin, Shiqiang.,Wang, Huaibin.,...&Yang, Wei.(2019).Structures and operating principles of the replisome.SCIENCE,363(6429),835-+. |
MLA | Gao, Yang,et al."Structures and operating principles of the replisome".SCIENCE 363.6429(2019):835-+. |
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