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DOI | 10.1126/science.aao0076 |
Rhomboid distorts lipids to break the viscosity-imposed speed limit of membrane diffusion | |
Kreutzberger, Alex J. B.1; Ji, Ming1; Aaron, Jesse2; Mihaljevic, Ljubica1; Urban, Sinisa1,2 | |
2019-02-01 | |
发表期刊 | SCIENCE
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ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2019 |
卷号 | 363期号:6426页码:497-+ |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | Enzymes that cut proteins inside membranes regulate diverse cellular events, including cell signaling, homeostasis, and host-pathogen interactions. Adaptations that enable catalysis in this exceptional environment are poorly understood. We visualized single molecules of multiple rhomboid intramembrane proteases and unrelated proteins in living cells (human and Drosophila) and planar lipid bilayers. Notably, only rhomboid proteins were able to diffuse above the Saffman-Delbruck viscosity limit of the membrane. Hydrophobic mismatch with the irregularly shaped rhomboid fold distorted surrounding lipids and propelled rhomboid diffusion. The rate of substrate processing in living cells scaled with rhomboid diffusivity. Thus, intramembrane proteolysis is naturally diffusion-limited, but cells mitigate this constraint by using the rhomboid fold to overcome the "speed limit" of membrane diffusion. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000457409400041 |
WOS关键词 | SINGLE-PARTICLE TRACKING ; LATERAL DIFFUSION ; GAMMA-SECRETASE ; PLASMA-MEMBRANE ; MOLECULE ; DYNAMICS ; PROTEIN ; RECEPTOR ; CELL ; CHANNELS |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/200673 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Johns Hopkins Univ, Sch Med, Dept Mol Biol & Genet, Room 507 PCTB,725 North Wolfe St, Baltimore, MD 21205 USA; 2.Howard Hughes Med Inst, Adv Imaging Ctr, Janelia Res Campus,19700 Helix Dr, Ashburn, VA 20147 USA |
推荐引用方式 GB/T 7714 | Kreutzberger, Alex J. B.,Ji, Ming,Aaron, Jesse,et al. Rhomboid distorts lipids to break the viscosity-imposed speed limit of membrane diffusion[J]. SCIENCE,2019,363(6426):497-+. |
APA | Kreutzberger, Alex J. B.,Ji, Ming,Aaron, Jesse,Mihaljevic, Ljubica,&Urban, Sinisa.(2019).Rhomboid distorts lipids to break the viscosity-imposed speed limit of membrane diffusion.SCIENCE,363(6426),497-+. |
MLA | Kreutzberger, Alex J. B.,et al."Rhomboid distorts lipids to break the viscosity-imposed speed limit of membrane diffusion".SCIENCE 363.6426(2019):497-+. |
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