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DOI | 10.1126/science.aav6740 |
Structure of the posttranslational Sec protein-translocation channel complex from yeast | |
Itskanov, Samuel1; Park, Eunyong2,3 | |
2019-01-04 | |
发表期刊 | SCIENCE
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ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2019 |
卷号 | 363期号:6422页码:84-+ |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | The Sec61 protein-conducting channel mediates transport of many proteins, such as secretory proteins, across the endoplasmic reticulum (ER) membrane during or after translation. Posttranslational transport is enabled by two additional membrane proteins associated with the channel, Sec63 and Sec62, but its mechanism is poorly understood. We determined a structure of the Sec complex (Sec61-Sec63-Sec71-Sec72) from Saccharomyces cerevisiae by cryo-electron microscopy (cryo-EM). The structure shows that Sec63 tightly associates with Sec61 through interactions in cytosolic, transmembrane, and ER-luminal domains, prying open Sec61's lateral gate and translocation pore and thus activating the channel for substrate engagement. Furthermore, Sec63 optimally positions binding sites for cytosolic and luminal chaperones in the complex to enable efficient polypeptide translocation. Our study provides mechanistic insights into eukaryotic posttranslational protein translocation. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000455315800066 |
WOS关键词 | ENDOPLASMIC-RETICULUM MEMBRANE ; MAMMALIAN SEC61 ; CRYO-EM ; TRANSPORT ; INSERTION ; VALIDATION ; MECHANISMS ; BINDING |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/200422 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Univ Calif Berkeley, Biophys Grad Program, Berkeley, CA 94720 USA; 2.Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA; 3.Univ Calif Berkeley, Calif Inst Quantitat Biosci, Berkeley, CA 94720 USA |
推荐引用方式 GB/T 7714 | Itskanov, Samuel,Park, Eunyong. Structure of the posttranslational Sec protein-translocation channel complex from yeast[J]. SCIENCE,2019,363(6422):84-+. |
APA | Itskanov, Samuel,&Park, Eunyong.(2019).Structure of the posttranslational Sec protein-translocation channel complex from yeast.SCIENCE,363(6422),84-+. |
MLA | Itskanov, Samuel,et al."Structure of the posttranslational Sec protein-translocation channel complex from yeast".SCIENCE 363.6422(2019):84-+. |
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