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DOI | 10.1126/science.aav7271 |
Functionally diverse type V CRISPR-Cas systems | |
Yan, Winston X.1; Hunnewell, Pratyusha1; Alfonse, Lauren E.1; Carte, Jason M.1; Keston-Smith, Elise1; Sothiselvam, Shanmugapriya1; Garrity, Anthony J.1; Chong, Shaorong1; Makarova, Kira S.2; Koonin, Eugene V.2; Cheng, David R.1; Scott, David A.1 | |
2019-01-04 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2019 |
卷号 | 363期号:6422页码:88-+ |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | Type V CRISPR-Cas systems are distinguished by a single RNA-guided RuvC domain-containing effector, Cas12. Although effectors of subtypes V-A (Cas12a) and V-B (Cas12b) have been studied in detail, the distinct domain architectures and diverged RuvC sequences of uncharacterized Cas12 proteins suggest unexplored functional diversity. Here, we identify and characterize Cas12c, -g, -h, and -i. Cas12c, -h, and -i demonstrate RNA-guided double-stranded DNA (dsDNA) interference activity. Cas12i exhibits markedly different efficiencies of CRISPR RNA spacer complementary and noncomplementary strand cleavage resulting in predominant dsDNA nicking. Cas12g is an RNA-guided ribonuclease (RNase) with collateral RNase and single-strand DNase activities. Our study reveals the functional diversity emerging along different routes of type V CRISPR-Cas evolution and expands the CRISPR toolbox. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000455315800067 |
WOS关键词 | NUCLEIC-ACID DETECTION ; CLASSIFICATION ; ENDONUCLEASE ; EVOLUTION ; COMPLEX ; CPF1 |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/200421 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Arbor Biotechnol, Cambridge, MA 02139 USA; 2.NIH, Natl Ctr Biotechnol Informat, Natl Lib Med, Bethesda, MD 20894 USA |
推荐引用方式 GB/T 7714 | Yan, Winston X.,Hunnewell, Pratyusha,Alfonse, Lauren E.,et al. Functionally diverse type V CRISPR-Cas systems[J]. SCIENCE,2019,363(6422):88-+. |
APA | Yan, Winston X..,Hunnewell, Pratyusha.,Alfonse, Lauren E..,Carte, Jason M..,Keston-Smith, Elise.,...&Scott, David A..(2019).Functionally diverse type V CRISPR-Cas systems.SCIENCE,363(6422),88-+. |
MLA | Yan, Winston X.,et al."Functionally diverse type V CRISPR-Cas systems".SCIENCE 363.6422(2019):88-+. |
条目包含的文件 | 条目无相关文件。 |
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