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DOI | 10.1126/science.aat1839 |
ATP-dependent force generation and membrane scission by ESCRT-III and Vps4 | |
Schoneberg, Johannes1,2,3; Pavlin, Mark Remec2,4; Yan, Shannon1,2; Righini, Maurizio6,10; Lee, Il-Hyung1,2,11; Carlson, Lars-Anders1,2,12; Bahrami, Amir Houshang3; Goldman, Daniel H.2,5,6,13; Ren, Xuefeng1,2; Hummer, Gerhard3,7; Bustamante, Carlos1,2,4,5,6,8,9; Hurley, James H.1,2,4,9 | |
2018-12-21 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2018 |
卷号 | 362期号:6421页码:1423-+ |
文章类型 | Article |
语种 | 英语 |
国家 | USA; Germany; Sweden |
英文摘要 | The endosomal sorting complexes required for transport (ESCRTs) catalyze reverse topology scission from the inner face of membrane necks in HIV budding, multivesicular endosome biogenesis, cytokinesis, and other pathways. We encapsulated ESCRT-III subunits Snf7, Vps24. and Vps2 and the AAA+ ATPase (adenosine triphosphatase) Vps4 in giant vesicles from which membrane nanotubes reflecting the correct topology of scission could be pulled. Upon ATP release by photo-uncaging, this system generated forces within the nanotubes that led to membrane scission in a manner dependent upon Vps4 catalytic activity and Vps4 coupling to the ESCRT-III proteins. Imaging of scission revealed Snf7 and Vps4 puncta within nanotubes whose presence followed ATP release, correlated with force generation and nanotube constriction, and preceded scission. These observations directly verify long-standing predictions that ATP-hydrolyzing assemblies of ESCRT-Ill and Vps4 sever membranes. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000453845000063 |
WOS关键词 | PROTEIN RECRUITMENT ; COMPLEX ; DYNAMICS ; FISSION ; DRIVEN ; CONSTRICTION ; RECOGNITION ; ASSEMBLIES ; ABSCISSION ; FILAMENTS |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/200377 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Univ Calif Berkeley, Dept Mol & Cell Biol, 229 Stanley Hall, Berkeley, CA 94720 USA; 2.Univ Calif Berkeley, Calif Inst Quantitat Biosci, Berkeley, CA 94720 USA; 3.Max Planck Inst Biophys, Dept Theoret Biophys, D-60438 Frankfurt, Germany; 4.Univ Calif Berkeley, Grad Grp Blophys, Berkeley, CA 94720 USA; 5.Univ Calif Berkeley, Howard Hughes Med Inst, Berkeley, CA 94720 USA; 6.Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA; 7.Goethe Univ, Inst Biophys, Frankfurt, Germany; 8.Univ Calif Berkeley, Dept Phys, Berkeley, CA 94720 USA; 9.Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging Div, Berkeley, CA 94720 USA; 10.Centrill Technol, Palo Alto, CA USA; 11.Univ Puget Sound, Dept Chem, Tacoma, WA 98416 USA; 12.Umea Univ, Wallenberg Ctr Mol Med, Dept Med Biochem & Biophys, Umea, Sweden; 13.Johns Hopkins Univ, Sch Med, Dept Mol Biol & Genet, Baltimore, MD 21205 USA |
推荐引用方式 GB/T 7714 | Schoneberg, Johannes,Pavlin, Mark Remec,Yan, Shannon,et al. ATP-dependent force generation and membrane scission by ESCRT-III and Vps4[J]. SCIENCE,2018,362(6421):1423-+. |
APA | Schoneberg, Johannes.,Pavlin, Mark Remec.,Yan, Shannon.,Righini, Maurizio.,Lee, Il-Hyung.,...&Hurley, James H..(2018).ATP-dependent force generation and membrane scission by ESCRT-III and Vps4.SCIENCE,362(6421),1423-+. |
MLA | Schoneberg, Johannes,et al."ATP-dependent force generation and membrane scission by ESCRT-III and Vps4".SCIENCE 362.6421(2018):1423-+. |
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