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DOI | 10.1126/science.aau8872 |
Structure of human TFIID and mechanism of TBP loading onto promoter DNA | |
Patel, Avinash B.1,2; Louder, Robert K.1,2,9; Greber, Basil J.2,3; Grunberg, Sebastian4,10; Luo, Jie5; Fang, Jie6; Liu, Yutong7; Banish, Jeff5; Hahn, Steve4; Nogales, Eva1,2,6,8 | |
2018-12-21 | |
发表期刊 | SCIENCE
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ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2018 |
卷号 | 362期号:6421页码:1376-+ |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | The general transcription factor IID (TFIID) is a critical component of the eukaryotic transcription preinitiation complex (PIC) and is responsible for recognizing the core promoter DNA and initiating PIC assembly. We used cryo-electron microscopy, chemical cross-linking mass spectrometry, and biochemical reconstitution to determine the complete molecular architecture of TFIID and define the conformational landscape of TFIID in the process of TATA box-binding protein (TBP) loading onto promoter DNA. Our structural analysis revealed five structural states of TFIID in the presence of TFIIA and promoter DNA, showing that the initial binding of TFIID to the downstream promoter positions the upstream DNA and facilitates scanning of TBP for a TATA box and the subsequent engagement of the promoter. Our findings provide a mechanistic model for the specific loading of TBP by TFIID onto the promoter. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000453845000050 |
WOS关键词 | RNA-POLYMERASE-II ; TATA-BINDING PROTEIN ; CORE PROMOTER ; CRYSTAL-STRUCTURE ; CRYO-EM ; TRANSCRIPTION COACTIVATOR ; EVOLUTIONARY CONSERVATION ; MOLECULAR RECOGNITION ; HISTONE H3 ; COMPLEX |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/200368 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Univ Calif Berkeley, Biophys Grad Grp, Berkeley, CA 94720 USA; 2.Lawrence Berkeley Natl Lab, Mol Biophys & Integrat BioImaging Div, Berkeley, CA 94720 USA; 3.Univ Calif Berkeley, Calif Inst Quantitat Biol QB3, Berkeley, CA 94720 USA; 4.Fred Hutchinson Canc Res Ctr, Div Basic Sci, Seattle, WA 98109 USA; 5.Inst Syst Biol, Seattle, WA 98109 USA; 6.Univ Calif Berkeley, Howard Hughes Med Inst, Berkeley, CA 94720 USA; 7.Univ Calif Berkeley, Dept Chem & Bimol Engn, Berkeley, CA 94720 USA; 8.Univ Calif Berkeley, Dept Mol & Cell Biol, 229 Stanley Hall, Berkeley, CA 94720 USA; 9.Johns Hopkins Univ, Dept Biol, Baltimore, MD 21218 USA; 10.New England Biolabs Inc, Ipswich, MA 01938 USA |
推荐引用方式 GB/T 7714 | Patel, Avinash B.,Louder, Robert K.,Greber, Basil J.,et al. Structure of human TFIID and mechanism of TBP loading onto promoter DNA[J]. SCIENCE,2018,362(6421):1376-+. |
APA | Patel, Avinash B..,Louder, Robert K..,Greber, Basil J..,Grunberg, Sebastian.,Luo, Jie.,...&Nogales, Eva.(2018).Structure of human TFIID and mechanism of TBP loading onto promoter DNA.SCIENCE,362(6421),1376-+. |
MLA | Patel, Avinash B.,et al."Structure of human TFIID and mechanism of TBP loading onto promoter DNA".SCIENCE 362.6421(2018):1376-+. |
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