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DOI | 10.1126/science.aau2599 |
High-affinity allergen-specific human antibodies cloned from single IgE B cell transcriptomes | |
Croote, Derek1; Darmanis, Spyros2; Nadeau, Kari C.3,4,5; Quake, Stephen R.1,2,6 | |
2018-12-14 | |
发表期刊 | SCIENCE
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ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2018 |
卷号 | 362期号:6420页码:1306-+ |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | Immunoglobulin E (IgE) antibodies protect against helminth infections but can also cause life-threatening allergic reactions. Despite their role in human health, the cells that produce these antibodies are rarely observed and remain enigmatic. We isolated single IgE B cells from individuals with food allergies and used single-cell RNA sequencing to elucidate the gene expression and splicing patterns unique to these cells. We identified a surprising example of convergent evolution in which IgE antibodies underwent identical gene rearrangements in unrelated individuals. Through the acquisition of variable region mutations, these IgE antibodies gained high affinity and unexpected cross-reactivity to the clinically important peanut allergens Ara h 2 and Ara h 3. These findings provide insight into IgE B cell transcriptomics and enable biochemical dissection of this antibody class. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000452994400056 |
WOS关键词 | RNA-SEQ ; TRANSMEMBRANE ; RESPONSES ; SEQUENCE ; REVEALS |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/200312 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Stanford Univ, Dept Bioengn, Stanford, CA 94305 USA; 2.Chan Zuckerberg Biohub, San Francisco, CA 94158 USA; 3.Stanford Univ, Sean N Parker Ctr Allergy & Asthma Res, Stanford, CA 94305 USA; 4.Stanford Univ, Dept Med, Stanford, CA 94305 USA; 5.Stanford Univ, Dept Pediat, Stanford, CA 94305 USA; 6.Stanford Univ, Dept Appl Phys, Stanford, CA 94305 USA |
推荐引用方式 GB/T 7714 | Croote, Derek,Darmanis, Spyros,Nadeau, Kari C.,et al. High-affinity allergen-specific human antibodies cloned from single IgE B cell transcriptomes[J]. SCIENCE,2018,362(6420):1306-+. |
APA | Croote, Derek,Darmanis, Spyros,Nadeau, Kari C.,&Quake, Stephen R..(2018).High-affinity allergen-specific human antibodies cloned from single IgE B cell transcriptomes.SCIENCE,362(6420),1306-+. |
MLA | Croote, Derek,et al."High-affinity allergen-specific human antibodies cloned from single IgE B cell transcriptomes".SCIENCE 362.6420(2018):1306-+. |
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